Identification and cluster analysis of non-O1/O139 Vibrio cholerae by MALDI-TOF MS
10.13602/j.cnki.jcls.2025.03.01
- VernacularTitle:MALDI-TOF MS对非O1/O139群霍乱弧菌的鉴定及聚类分析
- Author:
Maosuo XU
1
;
Hui ZHANG
;
Cong ZHOU
;
Chunmei SHEN
;
Yong LIN
Author Information
1. 复旦大学附属上海市第五人民医院,检验科,上海 200240
- Publication Type:Journal Article
- Keywords:
matrix-assisted laser desorption/ionization time-of-flight mass spectrometry;
non-O1/O139 Vibrio cholerae;
principal com-ponent analysis
- From:
Chinese Journal of Clinical Laboratory Science
2025;43(3):161-166
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify and cluster non-O1/O139 Vibrio cholerae(NOVC)using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF MS),and evaluate the feasibility of MALDI-TOF MS as a method for the identification and clustering of NOVC.Methods The NOVC was identified by the MALDI-TOF MS equipped with V5 database,V12 database,highly pathogenic bacteria database,and V5 database combined with self-built spectrum projection and analyzed by the principal com-ponent analysis(PCA)and main spectrum projection(MSP)clustering.Results The NOVC was incorrectly identified as Vibrio al-bensis by the MALDI-TOF MS equipped with V5 database or V12 database,while the MALDI-TOF MS equipped with highly pathogenic bacteria database or V5 database combined with self-built spectrum projection could correctly identify NOVC.The PCA clustering of MALDI-TOF MS could distinguish NOVC from other bacterial strains and refine the differentiation of NOVC species to show the dis-tance relationship between NOVC species.Some spectrum projections of NOVC were extremely similar to the reference strains used to establish the database,and MSP clustering could not distinguish the differences between NOVC species.Conclusion The identifica-tion ability of MALDI-TOF MS for NOVC is limited by its database.The MALDI-TOF MS equipped with highly pathogenic bacteria da-tabase or V5 database combined with the self-built spectrum projection can accurately identify NOVC.The PCA clustering of MALDI-TOF MS has certain reference significance for the intra-and inter-species identification and homology analysis of NOVC.
