Exploration on the Effects of Tuina on Glutamate Content and Synaptic Ultrastructure in Spinal Dorsal Horn of Rats with Chronic Sciatic Nerve Compression Injury Based on the SNAP25/VGLUT2 Pathway
10.19879/j.cnki.1005-5304.202408071
- VernacularTitle:基于SNAP25/VGLUT2通路探讨推拿对坐骨神经慢性压迫损伤大鼠脊髓背角谷氨酸含量及突触超微结构的影响
- Author:
Jingjing JIANG
1
;
Limei HUANG
;
Hongye HUANG
;
Hengchang CAI
;
Huanzhen ZHANG
;
Lechun CHEN
;
Shuijin CHEN
;
Shiye WU
;
Hui LIN
;
Zhigang LIN
Author Information
1. 福建中医药大学附属康复医院,福建 福州 350003;福建省康复技术重点实验室,福建 福州 350003
- Publication Type:Journal Article
- Keywords:
lumbar disc herniation;
tuina;
spinal dorsal horn;
SNAP25/VGLUT2 pathway;
glutamate;
synaptic ultrastructure;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2025;32(4):113-119
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of tuina on glutamate content and synaptic ultrastructure in spinal dorsal horn of rats with chronic sciatic nerve compression injury;To explore the potential mechanism of tuina regulation of the SNAP25/VGLUT2 pathway in alleviating lumbar disc herniation.Methods A chronic sciatic nerve compression injury model was used to simulate neuropathic pain in lumbar disc herniation.24 SD rats were randomly divided into blank group,model group and tuina group,with 8 rats in each group.From the 4th day after modeling,the tuina group was intervened with the tuina method for 10 minutes once a day for 14 consecutive days.The paw withdrawal threshold(PWT)and paw withdrawal latency(PWL)of rats in each group on the day before modeling,and the 4th,10th,14th and 17th days after modeling were detected.The spinal cord tissue of the modeling side was taken,synaptic ultrastructure of spinal dorsal horn neurons was observed using transmission electron microscopy,immunofluorescence staining was used to detect the expression of NR2A in the spinal dorsal horn,Western blot was used to detect the expression of SNAP25 protein in the spinal dorsal horn,immunohistochemistry was used to detect the expression of VGLUT2 in the spinal dorsal horn,ELISA was used to detect the content of glutamate in the spinal dorsal horn.Results Compared with the blank group,PWT and PWL of the model group were significantly reduced on the 4th,10th,14th and 17th days after modeling(P<0.001),with accumulation of vesicles in the presynaptic membrane of the dorsal horn of the spinal cord,increase in the area of the postsynaptic dense zone,and enlargement of the synaptic cleft,while the protein expressions of NR2A,SNAP25 and VGLUT2 in the spinal dorsal horn increased(P<0.05,P<0.001),and the content of glutamate increased(P<0.001).Compared with the model group,PWT and PWL of the tuina group rats significantly increased on the 10th,14th and 17th days after modeling(P<0.001),synaptic vesicles were evenly distributed,the area of the postsynaptic dense zone decreased,and the synaptic cleft decreased,while the protein expressions of NR2A,SNAP25 and VGLUT2 in the spinal dorsal horn decreased(P<0.05,P<0.001),and the content of glutamate decreased(P<0.01).Conclusion Tuina may regulate the content of glutamate through the SNAP25/VGLUT2 pathway in the spinal dorsal horn,improve the synaptic ultrastructure of neurons,and have an analgesic effect on lumbar disc herniation.
