Genomic characterization of a respiratory syncytial virus BA9 genotype clinical strain associated with a severe pneumonia outbreak
10.3760/cma.j.cn112309-20241022-00357
- VernacularTitle:1株导致重症肺炎暴发的呼吸道合胞病毒BA9基因型临床株的全基因组序列特征研究
- Author:
Na WANG
1
;
Jinhua SONG
1
;
Jie JIANG
1
;
Jingjing SONG
1
;
Yuqing SHI
1
;
Yan ZHANG
1
Author Information
1. 中国疾病预防控制中心病毒病预防控制所，传染病溯源预警与智能决策全国重点实验室，国家卫生健康委医学病毒和病毒病重点实验室，北京 102206
- Publication Type:Journal Article
- Keywords:
Respiratory syncytial virus;
BA9 genotype;
Whole-genome sequencing;
Genetic variation analysis
- From:
Chinese Journal of Microbiology and Immunology
2025;45(9):733-739
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the genomic characteristics of a clinical strain of respiratory syncytial virus (RSV) causing a severe pneumonia outbreak in a maternity center in a city in northern China in 2021.Methods:The whole genome sequence of RSV from a clinical sample obtained from a child with respiratory failure and heart failure was determined and analyzed using Sanger sequencing method. Sequence splicing and alignment were performed using bioinformatics software such as Sequencher 5.0, MEGA 7.0, and BioEdit 7.0. Besides, its phylogenetic relationship with the representative strains of RSV-B genotype, amino acid variations, and glycosylation sites were analyzed.Results:The genome of the clinical strain (named RSV/SY/2021) was 15 242 bp in length, consisting of 10 genes encoding 11 proteins, and possessed all the structural features of RSV. Phylogenetic analysis showed that the RSV/SY/2021 strain belonged to BA9 genotype and had the closest genetic relationship with the 2018 Netherlands epidemic strain MZ515558/Netherlands/2018. The termination codon mutation at the end of its attachment glycoprotein (G) gene resulted in the elongation of seven amino acids "Q-R-L-Q-S-Y-A" and the introduction of two additional O-glycosylation sites.Conclusions:This study suggests that RSV BA9 genotype may cause severe clinical symptoms, and clarifies the genome-wide characteristics and nucleotide/amino acid variation patterns of the RSV/SY/2021 strain. These findings enrich both national and global genome databases of RSV, and provide crucial etiological data for tracking RSV transmission, nucleic acid testing, and the development and evaluation of vaccines, antibodies and drugs against RSV.
