Neuronal Differentiation and Proliferation Inhibition of Human Glioma Cell A172 Induced by Clozapine
10.11969/j.issn.1673-548X.2025.03.023
- VernacularTitle:氯氮平诱导人脑胶质瘤细胞A172神经元样分化并抑制其增殖
- Author:
Zhaoqi TANG
1
;
Zhenhui CHEN
;
Chang CAO
Author Information
1. 361015 复旦大学附属中山医院厦门医院药剂科;361015 厦门市恶性肿瘤综合治疗临床医学研究中心
- Publication Type:Journal Article
- Keywords:
Clozapine;
Glioma;
Differentiation;
Neuron;
Proliferation
- From:
Journal of Medical Research
2025;54(3):126-131
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of clozapine on neuronal differentiation and proliferation of human glioma cells.Methods Hematoxylin-eosin(HE)staining was used to observe the effect of clozapine on the morphology of human glioma cell A172.Immunocytochemistry was used to detect the expression of neuronal markers neuronal nuclei antigen(NeuN)and microtubule-associated protein 2(MAP2),as well as the expression of astrocytic markers glial fibrillary acidic protein(GFAP)and S100 calcium binding protein B(S100B),and oligodendrocytic markers myelin basic protein(MBP)and Olig2.ki-67detection,CCK-8 assay and clone formation assay were used to assess the effects of clozapine on the cell proliferation of A172.SwissTargetPrediction,GeneCards,and STRING data-bases were utilized to predict the potential mechanism of clozapine.Western blot was used to detect the expression level of β-catenin.Results Clozapine(30μmol/L)changed the morphology of A172 cells,with elongated cell projection.Clozapine induced the expression of neuronal markers NeuN and MAP2 in A172 cells,without inducing the expression of astrocytic or oligodendrocytic markers.Clozapine significantly inhibited the proliferation of A172 cells.Database analysis suggested that the mechanism of clozapine might be related to the Wnt signaling pathway.Clozapine down-regulated the expression of β-catenin in A172 cells.Conclusion Clozapine induced neuronal differentiation of human glioma cells A172,and inhibited their proliferation.
