Agaricus blazei extract FA-2-b-β induces ferroptosis of Burkitt lympho-ma cells through STAT3/GPX4 signaling pathway
10.3969/j.issn.1000-4718.2025.03.005
- VernacularTitle:秦巴硒菇提取物FA-2-b-β通过STAT3/GPX4信号通路诱导伯基特淋巴瘤细胞铁死亡
- Author:
Jia WEI
1
;
Rong LI
;
Huiyan WANG
;
Zujun XI
;
Yanqing SUN
Author Information
1. 甘肃中医药大学中西医结合学院,甘肃 兰州 730030;甘肃省人民医院血液内科,甘肃 兰州 730030
- Publication Type:Journal Article
- Keywords:
Agaricus blazei Murill;
FA-2-b-β;
Burkitt lymphoma;
ferroptosis;
STAT3/GPX4 signaling pathway
- From:
Chinese Journal of Pathophysiology
2025;41(3):453-462
- CountryChina
- Language:Chinese
-
Abstract:
AIM:This study aims to investigate the effect of Agaricus blazei extract FA-2-b-β on ferroptosis of Burkitt lymphoma cells and its mechanism.METHODS:Burkitt lymphoma cell lines Raji and CA46 were treated with FA-2-b-β alone and in combination with ferrostatin-1,a ferroptosis inhibitor,or Stattic,a signal transducer and activator of transcription 3(STAT3)inhibitor.Cell viability was assessed using the CCK-8 method,and the half-maximal inhibitory concentration(IC50)of FA-2-b-β was calculated.Flow cytometry was used to detect apoptosis,cell cycle,mitochondrial membrane potential,and reactive oxygen species(ROS).Additionally,malondialdehyde(MDA)and glutathione(GSH)levels were measured using kits.The mRNA and protein expression levels of ferroptosis-related molecules were determined by RT-qPCR and Western blot.RESULTS:The extract FA-2-b-β at different concentrations significantly inhibited the proliferation of Raji and CA46 cells(P＜0.05),promoted their death,regulated cell arrest in G0/G1 phase,and decreased the mitochondrial membrane potential.(2)ROS and MDA levels were significantly increased with different concentrations of the extract FA-2-b-β(P＜0.05),while the GSH content was significantly decreased(P＜0.05).(3)The protein and mRNA levels of signal transducer and activator of transcription 3(STAT3),p-STAT3,and glutathione peroxidase 4(GPX4)were down-regulated at different concentrations of the extract FA-2-b-β.In addition,prostaglandin-endoperoxide synthase 2(PTSG2)and transferrin receptor protein 1(TfR1)protein and mRNA were up-regulated(P＜0.05),while the protein and mRNA levels of solute carrier family 7 member 11(SLC7A11)were not significantly changed.CONCLU-SION:The extract FA-2-b-β can induce ferroptosis in burkitt lymphoma,and the mechanism may be related to the inhibi-tion of STAT3/GPX4 signaling pathway.
