Chemical constituents from the stems and leaves of Dendrobium formosum and their biological activities
10.3969/j.issn.1001-1528.2025.02.017
- VernacularTitle:美丽石斛茎叶化学成分及其生物活性研究
- Author:
Li-hang CHI
1
;
Hui-qin CHEN
;
Sheng-zhuo HUANG
;
Fei WU
;
Wen-li MEI
;
Xi-qiang SONG
;
Hao-fu DAI
Author Information
1. 海南大学热带农林学院热带特色林木花卉遗传与种质创新教育部重点实验室,海南海口 570228;中国热带农业科学院热带生物技术研究所,海南省黎药资源天然产物研究与利用重点实验室,海南海口 571101
- Publication Type:Journal Article
- Keywords:
Dendrobium formosum Roxb.ex Lindl.;
stems and leaves;
chemical constituents;
isolation and identification;
α-glucosidase inhibitory activity;
in vitro anti-inflammatory activity;
PNPG method;
RAW264.7 cells
- From:
Chinese Traditional Patent Medicine
2025;47(2):467-473
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the chemical constituents from the stems and leaves of Dendrobium formosum Roxb.ex Lindl.and their biological activities.METHODS The 95％ethanol extract from the stems and leaves of D.formosum was isolated and purified by silica gel,Sephadex LH-20 and semi-preparative HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.Their inhibitory activities onα-glucosidase were determined by PNPG method,and their in vitro anti-inflammatory activities were evaluated by RAW264.7 model.RESULTS Fifteen compounds were isolated and identified as coniferyl p-coumarate(1),(-)-pinoresinol(2),2,5,7-trihydroxy-4-methoxy-9,10-dihydrophenanthrene(3),naringenin(4),spiropreussomerin A(5),7-hydroxy-14-de-O-methyl-lasiodiplodin(6),(4S,5S,6Z,8E)-5-hydroxydeca-6,8-dien-4-olide(7),(6S,9R)-blumenol C(8),p-hydroxybenzoic acid(9),m-hydroxybenzoic acid(10),p-hydroxy benzenepropanoic acid(11),5,7-dihydroxy-isobenzofuran(12),2-(4-hydroxyphenyl)-ethanol(13),β-sitostenone(14),β-sitosterol(15).The IC50 values of compounds 1 and 4 on α-glucosidase inhibition were(65.60±3.31)and(98.95±2.53)μmol/L,respectively.Compound 3 presented inhibitory activity on NO production in RAW 264.7 cells,with IC50 value of(3.97±0.12)μmol/L.CONCLUSION Compounds 5-6,8 and 12 are isolated from Orchidacae family for the first time,and 2-15 are first isolated from this plant.Compounds 1 and 4 have α-glucosidase inhibitory activities,and 3 has anti-inflammatory activity.
