Simultaneous content determination of twenty-one constituents in Huangqi Guizhi Wuwu Decoction by HPLC-MS/MS
10.3969/j.issn.1001-1528.2025.02.002
- VernacularTitle:HPLC-MS/MS法同时测定黄芪桂枝五物汤中21种成分的含量
- Author:
Qiu-gu CHEN
1
;
Jin-ru WU
1
;
Chang-hui LI
1
;
Shang-bin ZHANG
1
;
Yuan ZHAO
1
;
Jian-ping CHEN
1
Author Information
1. 广州中医药大学第四临床医学院深圳市中医院,深圳市医院中药制剂研究重点实验室,广东 深圳 518033
- Publication Type:Journal Article
- Keywords:
Huangqi Guizhi Wuwu Decoction;
chemical constituents;
content determination;
HPLC-MS/MS
- From:
Chinese Traditional Patent Medicine
2025;47(2):365-371
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish an HPLC-MS/MS method for the simultaneous content determination of gallic acid,protocatechuic acid,oxypaeoniflorin,catechin,epicatechin,albiflorin,paeoniflorin,rutin,calycosin-7-glucoside,syringaldehyde,ferulic acid,coumarin,ononin,calycosin,cinnamic alcohol,cinnamic acid,benzoylpaeoniflorin,cinnamaldehyde,astragaloside,astragaloside Ⅲ,6-gingerol in Huangqi Guizhi Wuwu Decoction.METHODS The analysis was performed on a 30 ℃ thermostatic Thermo Scientific Hypersil GOLD column(150 mmx4.6 mm,3 μm),with the mobile phase comprising of 0.015％formic acid-acetonitrile flowing at 0.4 mL/min in a gradient elution manner,and electrospray ionization source was adopted in positive and negative ion modes with multiple reaction monitoring.RESULTS Twenty-one constituents showed good linear relationships within their own ranges(r＞0.990 5),whose average recoveries were 93.99％-108.52％with the RSDs of 1.04％-5.97％.CONCLUSION This simple,feasible,stable and reliable method can be used for the quality control of Huangqi Guizhi Wuwu Decoction.
