Comparison of active constituent contents and their biological activities of Buzhong Yiqi Recipe with different dosage forms
10.3969/j.issn.1001-1528.2025.02.001
- VernacularTitle:补中益气方不同剂型中活性成分含量及其生物活性比较
- Author:
Yan-ran HE
1
;
Jing WANG
;
Jia-qiang XU
;
Zhao-zhao XIA
;
Ying-jiao LIU
;
Zi-shu DONG
;
Liang-shan MING
;
Hong-ning LIU
;
Qi-meng FAN
Author Information
1. 江西中医药大学高等研究院,江西南昌 330004;江西中医药大学药学院,江西南昌 330004
- Publication Type:Journal Article
- Keywords:
Buzhong Yiqi Recipe;
dosage forms;
active constituents;
content determination;
antioxidant activities;
hypoglycemic activities
- From:
Chinese Traditional Patent Medicine
2025;47(2):357-364
- CountryChina
- Language:Chinese
-
Abstract:
AIM To compare total sugar,total protein,total phenol,total flavonoid,calycosin-7-O-β-D-glucoside,liquiritin,lobetyolin,quercetin,isoferulic acid,hesperidin,glycyrrhizic acid contents and their antioxidant activities,hypoglycemic activities of big honey pill,small honey pill,water pill,concentrated pill,granule,mixture and decoction of Buzhong Yiqi Recipe.METHODS Anthraquinone-sulfuric acid method,Coomassie brilliant blue method,Folin-phenol colorimetry method,sodium nitrite-aluminum nitrate method and HPLC were adopted in the content determination of total sugar,total protein,total phenol,total flavonoid and seven constituents,respectively,after which the scavenging capacities,reducing powers on DPPH·free radical,ABTS+free radical,hydroxyl free radical,and inhibition capacity on α-glucosidase activity were detected.Subsequently,correlation analysis was performed.RESULTS Total sugar,total protein,total phenol and total flavonoid contents demonstrated significant differences among different dosage forms(P＜0.05,P＜0.01).Calycosin-7-O-β-D-glucoside,glycyrrhizin,codonoside and quercetin displayed the highest contents in the decoction,while those of isoferulic acid,hesperidin and glycyrrhizin were observable in the mixture.The water pill exhibited the strongest antioxidant activity,while those of the concentrated pill and mixture were weak;the big honey pill exhibited the strongest hypoglycemic activity,while that of the decoction was the weakest.Total protein,total phenol,total flavonoid and liquiritin contents displayed significant positive correlations between antioxidant activity(P＜0.05,P＜0.01),while hesperidin content displayed significant negative correlation between the latter(P＜0.05);total protein,calycosin-7-O-β-D-glucoside,codonoside and quercetin contents displayed significant negative correlations between hypoglycemic activity(P＜0.05,P＜0.01).CONCLUSION Active constituent contents and their biological activities of Buzhong Yiqi Recipe with different dosage forms exist differences,total sugar,total protein,total flavonoids,calycosin-7-O-β-D-glucoside,licorice glycoside,hesperidin,codonoside and quercetin can be taken as quality control indices for this prescription.
