Intervention effect of salvianolic acid B based on JAK2/STAT3 pathways on rats with Escherichia coli infection-induced pyelonephritis
10.11816/cn.ni.2025-240920
- VernacularTitle:丹酚酸B基于JAK2/STAT3通路对大肠埃希菌感染肾盂肾炎大鼠的干预效果
- Author:
Ling ZHANG
1
;
Zhifang WANG
1
;
Jinxiao JIANG
1
;
Chen QI
1
;
Gufei HUANG
1
Author Information
1. 黄冈市中心医院肾病学科,湖北黄冈 438000
- Publication Type:Journal Article
- Keywords:
Salvianolic acid B;
Protein tyrosine kinase-2/signal transduction and transcriptional activator 3;
Esche-richia coli;
Pyelonephritis;
Infection
- From:
Chinese Journal of Nosocomiology
2025;35(6):807-811
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To observe intervention effect of salvianolic acid B based on JAK2/STAT3 pathways on the rats with Escherichia coli infection-induced pyelonephritis.METHODS Totally 60 SPF grade male SD rats were in-cluded in the study and were randomly divided into the control group with 21 rats,the model group(Escherichia coli infection-induced pyelonephritis)with 18 rats and the salvianolic acid B group(Escherichia coli infection-in-duced pyelonephritis+salvianolic acid B)with 18 rats.The control group and the model group were treated with same volume of normal saline for gavage,the salvianolic acid B group was given 12.5 mg/kg salvianolic acid B wa-ter solution for gavage.The levels of related biochemical indexes[secretory immunoglobulin A(SlgA),urine cre-atinine and serum creatinine]were detected for the groups.The organ indexes and relative expression levels of JAK2/STAT3 signaling pathways were observed and compared among the groups.RESULTS There were significant differences in the levels of blood indexes among the three groups of rats(P＜0.05).The SlgA level of the model group was(13.51±1.18)μg/ml,lower than(25.64±4.51)μg/ml of the control group(P＜0.05);the SlgA level of the salvian-olic acid B group was(25.34±2.95)μg/ml,higher than that of the model group(P＜0.05).There were no significant differences in the bladder indexes among the three groups;the ratio of left kidney to right kidney and the renal indexes were higher in the model group than in the control group(P＜0.05);the ratio of left kidney to right kidney and the renal indexes were lower in the salvianolic acid B group than in the model group(P＜0.05).There were significant differences in the relative expression levels of JAK2/STAT 3 signaling pathways among the three groups(P＜0.05);the levels of JAK2 and STAT 3 of the model group were higher than those of the control group(P＜0.05);the levels of JAK2 and STAT 3 of the salvianolic acid B group were lower than those of the model group(P＜0.05).CONCLUSION The salvian-olic acid B based on JAK2/STAT3 pathways may improve the indexes of the rats with E.coli infection-induced pyelonephritis and effectively improve the overall symptoms.
