Distribution and drug resistance of pathogens isolated from children with recurrent respiratory tract infections and expressions of TLRs pathways in peripheral blood mononuclear cells
10.11816/cn.ni.2025-240470
- VernacularTitle:儿童反复呼吸道感染病原菌和耐药性及外周血单核细胞TLRs通路表达水平
- Author:
Rong LIN
1
;
Qiu LIN
1
;
Qinghong YANG
1
;
Zhuling ZHENG
1
Author Information
1. 海南现代妇女儿童医院儿科,海南海口 570100
- Publication Type:Journal Article
- Keywords:
Recurrent respiratory tract infection in children;
Peripheral blood mononuclear cell;
Toll-like receptor pathway;
Pathogen;
Drug resistance;
Receiver operating characteristic curve
- From:
Chinese Journal of Nosocomiology
2025;35(6):895-899
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the distribution and drug resistance of pathogens isolated from the children with recurrent respiratory tract infection(RRI)and analyze the expressions of Toll-like receptors(TLRs)in peripheral blood mononuclear cells(PBMCs)so as to provide bases for the diagnosis.METHODS A total of 52 children with RRI who were treated in Hainan Modern Women and Children's Hospital from Oct.2021 to Oct.2023 were re-cruited as the case group,meanwhile,61 healthy children who received physical examination were chosen as the healthy group.The distribution and drug resistance of the pathogens isolated from the case group were statistically analyzed.The levels of PBMCs TLR2 and TLR4 were compared between the two groups.The values of TLR2 and TLR4 in diagnosis of the children with RRI were analyzed by means of receiver operating characteristic(ROC)curves.RESULTS Totally 311 strains of pathogens were isolated from the 52 children with RRI,among which gram-negative bacteria were dominant;Haemophilus influenzae was the major species of gram-negative bacteria,and Staphylococcus aureus was the predominant species of gram-positive bacteria.The H.influenzae strains were mainly resistant to ceftazidime,cefepime and gentamycin,but were sensitive to imipenem and meropenem.The S.aureus strains were highly resistant to penicillin and ampicillin,but were sensitive to vancomycin and nitrofuran-toin.There were significant differences in the levels of PBMCs TLR2 and TLR4 between the case group and the healthy group(P＜0.05).The area under the curve(AUC)value of the joint detection of TLR2 and TLR4 was higher than that of the single detection in diagnosis of the children with RRI(P＜0.05),the sensitivity of the joint detection was 76.92％,with the specificity 95.08％.CONCLUSIONS The gram-negative bacteria are dominant a-mong the pathogens isolated from the children with RRI.Imipenem and meropenem are recommended for the treatment of H.influenzae infection;vancomycin and nitrofurantoin are recommended for treatment of S.aureus infection.The children with RRI show the abnormal expressions of TLRs in PBMCs.The joint detection of TLR2 and TLR4 can raise the diagnostic value of the RRI children.
