Expression and significance of E2F1 in head and neck squamous cell carcinoma based on bioinformatics analysis
10.3969/j.issn.1001-3733.2025.02.017
- VernacularTitle:基于生物信息学分析E2F1在头颈鳞癌中的表达及临床意义
- Author:
Jiao LI
1
;
Ning AN
Author Information
1. 430000 武汉,华中科技大学同济医学院附属武汉儿童医院(武汉市妇幼保健院)口腔科
- Publication Type:Journal Article
- Keywords:
E2F1;
Head and neck squamous cell carcinoma;
Immunohistochemistry;
RT-PCR;
TCGA;
Bioinformatics
- From:
Journal of Practical Stomatology
2025;41(2):244-252
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression,clinical significance and prognostic relevance of transcription factor E2F1 in head and neck squamous cell carcinoma(HNSC)tissues.Methods:Based on the cancer genome atlas(TCGA)database,the tran-scriptional level of E2F1 in HNSC,its relationship with diagnostic value and survival of HNSC patients were analyzed.Correlation of E2F1 expression levels in HNSC and its clinicopathological parameters were analyzed by UALCAN database and GEPIA database.Related genes of E2F1 were analyzed by CBioportal database,the coresponding results were studied by KEGG/GO enrichment analy-sis.The expression of E2F1 mRNA in 44 cases of oral squamous cell carcinoma(OSCC)and 21 controls of normal oral mucosa tis-sue was detected by immunohistochemistry and PCR.Results:TCGA data revealed that E2F1 expression in HNSC was significantly higher than in normal tissue(P＜0.01),which was consistent with the results of IHC and PCR(both P＜0.01).ROC showed that the AUC was 0.966,E2F1 expression was positively correlated with the prognosis of HNSC patients(P＜0.05).PCR analysis showed that E2F1 mRNA level was high in OSCC and corelated with the differetation,clinical stage and limph metastasis(P＜0.05),and not with the age and gender(P＞0.05).Meanwhile,KEGG/GO enrichment analysis showed that biological processes of E2F1 and its related genes were mainly involved in mismatch repair,cell cycle,DNA replication and other processes.Conclusion:The high expression of E2F1 may be closely related to the occurrence,development and metastasis of HNSC.
