Effects of Congrong San on neuronal apoptosis and Bax/Bcl-2/Caspase3 signaling pathway in a rat model of Alzheimer's disease
10.3969/j.issn.1001-1528.2025.04.009
- VernacularTitle:苁蓉散对阿尔茨海默病大鼠神经元细胞凋亡和Bax/Bcl-2/Caspase3信号通路的影响
- Author:
Yuan-qin CAI
1
;
Yang XIANG
;
Qing-hua LONG
;
Xi WANG
;
Jing-fan ZHANG
;
Chu-hua ZENG
Author Information
1. 云南中医药大学,云南省教育厅中医药防治神经精神疾病重点实验室,云南 昆明 650500;湖北民族大学医学部,湖北恩施 445000
- Publication Type:Journal Article
- Keywords:
Congrong San;
Alzheimer's disease;
hippocampus;
neuronal damage;
apoptosis;
amyloid precursor protein(APP);
β-site amyloid precursor protein cleaving enzyme 1(BACE1)
- From:
Chinese Traditional Patent Medicine
2025;47(4):1122-1128
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the effects of Congrong San on neuronal apoptosis and the Bax/Bcl-2/Caspase3 signaling pathway in a rat model of Alzheimer's disease(AD).METHODS A total of 60 2-month-old SD male rats were randomly divided into the blank group,the model group,the memantine hydrochloride group(0.025 g/kg)and low-dose,medium-dose and high-dose Congrong San groups(4.62,9.24,18.48 g/kg).All groups except the control group received stereotactic intracerebral injection of Aβ1-42 to establish AD models.Following the successful modeling,each group received its corresponding intragastric administration once daily for 28 consecutive days.After the administration,the rats had their learning and memory ability detected by the morris water maze test;their hippocampal neuronal morphology observed with HE and Nissl staining;their hippocampal neuronal apoptosis observed with TUNEL staining;and their hippocampal expressions of amyloid precursor protein(APP),β-site APP-cleaving enzyme 1(BACE1),and apoptosis-related proteins Bax,Bcl-2 and Caspase3 detected with Western blot assay.RESULTS Compared with the model group,the groups intervened with medium-dose and high-dose Congrong San exhibited improved learning and memory performance,alleviated hippocampal neuronal damage,increased Nissl body count(P＜0.01),reduced hippocampal apoptosis rate(P＜0.05,P＜0.01),decreased protein expressions of APP,BACE1,Bax and cleaved-Caspase3/Caspase3 ratio(P＜0.05,P＜0.01),and elevated Bcl-2 expression(P＜0.01).CONCLUSION Congrong San mitigates cognitive impairment,hippocampal neuronal damage,and apoptosis in AD rats,probably through inhibition of the Bax/Bcl-2/Caspase3 signaling pathway activation.
