In vivo pharmacokinetics of five effective constituents in Asteris Radix et Rhizoma in rats
10.3969/j.issn.1001-1528.2025.04.006
- VernacularTitle:紫菀中5种有效成分在大鼠体内药动学研究
- Author:
Ling FAN
1
;
Ming-zhi WANG
;
Yan WU
;
Jia-mei GU
;
Ya-jie ZHAO
;
Xin WANG
Author Information
1. 苏州市中西医结合医院,江苏苏州 215101
- Publication Type:Journal Article
- Keywords:
Asteris Radix et Rhizoma;
effective constituents;
in vivo pharmacokinetics;
UPLC-MS/MS
- From:
Chinese Traditional Patent Medicine
2025;47(4):1104-1111
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the in vivo pharmacokinetics of chlorogenic acid,isoquercetin,ferulic acid,isorhamnetin and shionone in Asteris Radix et Rhizoma in rats.METHODS Twelve rats were randomly assigned into 2 groups and given intragastric administration of conventional dose(0.63 g/kg)and large dose(3.3 g/kg)of Asteris Radix et Rhizoma extracts,respectively,after which blood collection was made at 0.083,0.167,0.33,0.5,0.75,1,1.5,2,4,6,8,12,24 h,UPLC-MS/MS was adopted in the plasma concentration determination of various effective constituents,and main pharmacokinetic parameters were calculated.RESULTS Various effective constituents in the large dose group demonstrated prolonged t1/2,MRT0-∞ as compared with those in the conventional dose group(P＜0.05).After dose correction,Cmax of chlorogenic acid,isoquercetin,shionone in the large dose group displayed no obvious changes(P＞0.05),while Cmax of ferulic acid,isorhamnetin,and AUC0-t,AUC0-∞ of various effective constituents were higher than those in the conventional dose group(P＜0.05).CONCLUSION Various effective constituents in the high dose of Asteris Radix et Rhizoma can maintain high-concentration and long-time effects on rat bladder tissue.
