Quality evaluation of Rubi Fructus based on UPLC-Q-TOF-MS/MS,HPLC fingerprints and content determination
10.3969/j.issn.1001-1528.2025.04.002
- VernacularTitle:基于UPLC-Q-TOF-MS/MS法、HPLC指纹图谱、含量测定评价覆盆子质量
- Author:
Shu-wan LI
1
;
Yang-zhen LIAO
;
Long-fei WANG
;
Ya-qin ZHAO
;
Jia-he FAN
;
Yuan WEI
;
Zhen OUYANG
Author Information
1. 江苏大学药学院,江苏镇江 212013
- Publication Type:Journal Article
- Keywords:
Rubi Fructus;
quality evaluation;
UPLC-Q-TOF-MS/MS;
HPLC fingerprints;
content determination;
chemical pattern recognition
- From:
Chinese Traditional Patent Medicine
2025;47(4):1077-1084
- CountryChina
- Language:Chinese
-
Abstract:
AIM To evaluate the quality of Rubi Fructus.METHODS UPLC-Q-TOF-MS/MS was adopted in the component identification,after which the HPLC fingerprints were established,cluster analysis,principal component analysis and orthogonal partial least squares discriminant analysis were used for chemical pattern recognition.and the contents of chlorogenic acid,ferulic acid,ellagic acid,isoquercitrin,kaempferol-3-O-rutinoside,astragalin,tiliroside quercetin,kaempferol were determined.RESULTS Total 34 constituents were identified.There were 19 common peaks in the fingerprints for 31 batches of medicinal materials with the similarities of more than 0.8.Wild varieties and cultivated varieties,and medicinal materials from different producing areas could be distinguished;4 principal components demonstrated the accumulative variance contribution rate of 84.142％;8 differential components were screened,2 of which were ellagic acid and astragalin.Ellagic acid and astragalin displayed higher contents in the wild varieties than those in the cultivated varieties(P＜0.05,P＜0.01).CONCLUSION UPLC-Q-TOF-MS/MS,HPLC fingerprints combined with content determination can be used for the quality control of Rubi Fructus.
