Quality evaluation of Croci Stigma from different producing areas
10.3969/j.issn.1001-1528.2025.04.003
- VernacularTitle:不同产地西红花质量评价
- Author:
Rui-qi WANG
1
;
Yi-qi SHEN
1
;
XU CHEN SI-HAN
1
;
Yong ZHANG
1
;
Tong ZHANG
1
;
Yue DING
1
Author Information
1. 上海中医药大学,上海 201203
- Publication Type:Journal Article
- Keywords:
Croci Stigma;
producing areas;
quality evaluation;
UPLC fingerprints;
orthogonal partial least squares discriminant analysis;
content determination;
color;
Pearson correlation analysis
- From:
Chinese Traditional Patent Medicine
2025;47(4):1084-1091
- CountryChina
- Language:Chinese
-
Abstract:
AIM To evaluate the quality of Croci Stigma from different producing areas.METHODS The analysis was performed on a 25 ℃ thermostatic Waters Acquity UPLC HSS T3 column(2.1 mm× 100 mm,1.8μm),with the mobile phase comprising of 0.1％phosphoric acid-acetonitrile flowing at 0.35 mL/min in a gradient elution manner,and the detection wavelengths were set at 254,440 nm.The UPLC fingerprints were established,after which orthogonal partial least squares discriminant analysis was performed,picrocrocin,crocin-Ⅰ,crocin-Ⅱ,crocin-Ⅲ,crocin-Ⅳ contents and chromaticity values(L*,a*,b*,E*ab)were determined,Pearson correlation analysis was adopted in the investigation of correlations between chromaticity values and internal constituent contents.RESULTS There were 14 common peaks in the fingerprints for 22 batches of medicinal materials with the similarities of more than 0.98.Various batches of medicinal materials were clustered into 2 types,7 quality difference components were screened.crocin-Ⅰ content in medicinal materials from different producing areas demonstrated significant differences(P＜0.05);the redder the color of medicinal material,the higher the contents of crocins.Picrocrocin,crocin-Ⅰ,crocin-Ⅱ,crocin-Ⅳ contents displayed highly significant correlations with colorimetric values(P＜0.01),while crocin-Ⅲ content exhibited no significant correlation with the latter(P＞0.05).CONCLUSION This accurate and reliable method can provide references for the quality control and color-quality relationship elucidation of Croci Stigma.
