Effects of aqueous extract of Bulbophyllum kwangtungense on liver damage and Nrf2/HO-1/NQO-1 signaling pathway in a rat model of D-galactose-induced oxidative stress
10.3969/j.issn.1001-1528.2025.04.012
- VernacularTitle:广东石豆兰水提物对D-半乳糖致大鼠肝组织氧化损伤及Nrf2/HO-1/NQO-1信号通路的影响
- Author:
Bing-bing WANG
1
;
Hua ZHU
1
;
Miao ZHANG
1
;
Li-ba XU
1
;
Chun-he WEI
1
;
Ting HUANG
1
Author Information
1. 广西中医药大学,广西壮瑶药重点实验室,壮瑶药协同创新中心,广西壮族自治区民族药资源与应用工程研究中心,广西南宁 530200
- Publication Type:Journal Article
- Keywords:
Bulbophyllum kwangtungense Schltr.;
aqueous extract;
D-gal;
oxidative stress;
apoptosis;
Nrf2/HO-1/NQO-1 signaling pathway
- From:
Chinese Traditional Patent Medicine
2025;47(4):1143-1149
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the protective effects of the aqueous extract of Bulbophyllum kwangtungense Schltr.on D-galactose(D-gal),an oxidative stress-induced liver damage in rats.METHODS 60 Male SD rats were randomly divided into the normal control group,the model group,the resveratrol group(20 mg/kg),and low-dose,medium-dose,and high-dose B.kwangtungense aqueous extract groups(750,1 500 and 3 000 mg/kg).Except for those of the normal control group,the rats of the other groups were subcutaneously injected with D-gal(200 mg/kg)in the back of neck to establish a liver damage model.Simultaneously,each group underwent the corresponding drug administration by gavage(10 mL/kg)once daily for 42 days.After the treatment period,the rats had their peripheral blood and liver tissues collected for the calculation of the liver index;their serum AST and ALT activities,hepatic SOD and GSH-Px activities,and MDA levels measured;their hepatic pathological changes observed using HE staining;their hepatic mRNA expressions of Nrf2,NQO-1 and HO-1 detected by RT-qPCR;and their hepatic protein expressions of Nrf2,NQO-1,HO-1,Bax,Bcl-2 and cleaved Caspase-3 measured by Western blot.RESULTS Compared with the model group,the groups intervened with medium-dose or high-dose B.kwangtungense aqueous extract showed increased liver index level(P＜0.01);decreased serum AST and ALT activities(P＜0.01);increased hepatic SOD and GSH-Px activities(P＜0.05,P＜0.01);reduced MDA levels(P＜0.01);alleviated pathological liver damage and increased hepatic mRNA and protein expressions of Nrf2,NQO-1 and HO-1(P＜0.05,P＜0.01);and reduced protein expressions of cleaved Caspase-3 and the Bax/Bcl-2 ratio as well(P＜0.05,P＜0.01).CONCLUSION The aqueous extract of B.kwangtungense is protective of rats with D-gal-induced liver injury,and the underlying mechanism may associate with the inhibition of oxidative stress and the suppression of hepatocellular apoptosis mediated by the activation of the Nrf2/HO-1/NQO-1 signaling pathway.
