Chemical constituents from Tylophora ovata and their antibacterial activities
10.3969/j.issn.1001-1528.2025.04.017
- VernacularTitle:娃儿藤化学成分及其抑菌活性研究
- Author:
Xi-yue HE
1
;
Xiao-jiang HAO
;
Qi-long LIANG
;
Jun-you JIAN
;
Lie-jun HUANG
Author Information
1. 贵州医科大学药学院,贵州贵阳 550025;贵州医科大学省部共建药用植物功效与利用国家重点实验室,贵州贵阳 550014;贵州省天然产物研究中心,贵州贵阳 550002
- Publication Type:Journal Article
- Keywords:
Tylophora ovata(Lindl.)Hook.ex Steud.;
chemical constituents;
isolation and identification;
antibacterial activities;
mycelial growth rate method
- From:
Chinese Traditional Patent Medicine
2025;47(4):1172-1181
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the chemical constituents from Tylophora ovata(Lindl.)Hook.ex Steud.and their antibacterial activities.METHODS Ethanol extract was isolated and purified by MCI,silica gel,Sephadex LH-20 and semi-preparative HPLC,then the structures of obtained compounds were identified by spectral data.The inhibitory activities of each compound against Phomopsis sp.were determined by mycelial growth rate method.RESULTS Twenty-six compounds were identified as paeonol(1),stigmast-4-en-3-one(2),ergosta-4,6,8(14),22-tetraen-3-one(3),2,4-methoxyphenol(4),1,2,4-trimethoxybenzene(5),3-methoxyphenol(6),3,4-dimethoxyacetophenone(7),5α,8α-epidioxy-ergosta-6,22(E)-diene-3β-ol(8),kaempferol 3-O-β-D-galactopyranoside(9),glaucogenind C(10),glaucoge-nin A 3-O-β-D-cymaropyranoside(11),dibutyl phthalate(12),cynatratoside A(13),hirundigoside C(14),sublanceoside B2(15),cynanoside A(16),dipentyl phthalate(17),5-hydroxymethyl-2-furancarboxaldehyde(18),bis-(2-ethyl)hexylphthalate(19),p-hydroxybenzoic acid(20),syringic acid(21),β-hydroxypropiovanillone(22),3-hydroxy-l-(4-hydroxy-3,5-dimethoxyphenyl)-1-propanone(23),(+)-syringare sinol(24),(-)-syringare sinol(25),(+)-medioresinol(26).IC50 value of compound 12 was 37.27 μg/mL.CONCLUSION Compounds 1-26 are isolated from this plant for the first time.Compound 12 has inhibitory activity against Phomopsis sp.
