Detection and analysis of pathogens associated with neoplastic diseases in breeders in Hebei Province
10.16303/j.cnki.1005-4545.2025.03.26
- VernacularTitle:河北某种鸡场肿瘤病的病原检测及分析
- Author:
Xueyan YI
1
;
Runyu ZHOU
1
;
Heyu YIN
1
;
Yuhua GU
1
;
Huali KUANG
1
;
Yunyu LI
1
;
Qinghui JIA
1
;
Peiguo LI
1
Author Information
1. 河北科技师范学院动物科技学院河北省预防兽医学重点实验室,河北秦皇岛 066000
- Publication Type:Journal Article
- Keywords:
breeders;
tumor diseases;
pathogen analysis;
ALV-J gp85 gene;
MDV meq gene
- From:
Chinese Journal of Veterinary Science
2025;45(3):594-601
- CountryChina
- Language:Chinese
-
Abstract:
In order to determine the causative agents responsible for the lethality of tumor disease in breeders,liver,spleen and other tissues of dead chickens were collected,and pathogen detection,se-quencing and genetic evolution analysis were carried out by PCR.The results showed that all three samples were negative for reticuloendotheliosis virus(REV),samples 1 and 2 wereavian leukosis virus subgroup J(ALV-J)positive,and sample 3 was positive for ALV-J and Marek's disease virus(MDV),which preliminarily determined that the breeder died of ALV-J infection or ALV-J and MDV mixed infection.Among them,the ALV-J gp85 gene had the highest nucleotide homology with the reference strain of ALV-J,ranging from 87.70％to 99.30％,and was in the same branch with the reference strain of subgroup J.Amino acid homology ranged from 78.00％to 96.20％,with some mutations.The nucleotide homology between the MDV meq gene and the vv+strain was the highest,which was 98.00％-99.00％,and they were in the same branch.The homology of amino acids was 95.90％-98.20％,and there were multiple mutation sites,among which the 176th amino acid destroyed the original Pro repeat of the virus MDV meq due to the mutation of Pro to Arg or Ala,which may lead to the enhancement of its virulence.This study will provide a reference for the prevention and control of neoplastic diseases in breeders in this region.
