The effects of resveratrol on the level of autophagy in the spleen of mice with OSAHS
10.3969/j.issn.1001-3733.2025.02.010
- VernacularTitle:白藜芦醇对OSAHS小鼠脾脏自噬水平的影响
- Author:
Liru ZHAO
1
;
Guanzhou HOU
;
Bowen DAI
;
Xiaolei GE
;
Xiuge GU
;
Yang LIU
;
Linna WANG
;
Xinyu XIE
;
Jiaqi MAO
Author Information
1. 050017 石家庄,河北医科大学口腔医学院·口腔医院正畸科,河北省口腔医学重点实验室,河北省口腔疾病临床医学研究中心
- Publication Type:Journal Article
- Keywords:
OSAHS;
Hypoxia;
Autophagy;
Spleen;
Resveratrol
- From:
Journal of Practical Stomatology
2025;41(2):200-205
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of hypoxia on the autophagy level in the spleen of mice with OSAHS.Methods:Mouse OSAHS hypoxia model was established in 24 C57BL/6 mice and the mice were divided into 3 groups:normoxic group,hypoxic group and resveratrol group(n=8).Real time PCR,Western blot and immunohistochemical staining(IHC)were used to detect the mRNA and protein expression of autophagy related proteins microtube associated protein 1 light chain 3 B(LC3B),myosin-like BCL2 inteacting protein(Beclin1)and autophagy related gene 5(ATG5)in mouse spleen respectively.Results:The mRNA expres-sion of LC3B,Beclin1,and ATG5 in the hypoxic group was lower than that in the normoxic group(P＜0.01,P＜0.01,P＜0.001),resveratrol upregulated the expression levels of LC3B and ATG5 mRNA in the hypoxic group(P＜0.05,P＜0.001),but did not up-regulate the expression of Beclin1(P＞0.05).The protein expression levels of LC3B,Beclin1 and ATG5 in the hypoxic group were lower than that in the normoxic group(P＜0.05,P＜0.05,P＜0.01).Resveratrol upregulated the expression levels of LC3B and ATG5 in the hypoxic group(P＜0.05,P＜0.01),but did not upregulated the expression of Beclin1(P＞0.05).IHC test showed that the expression level of LC3B,Beclin1 and ATG5 in the hypoxic group was lower than that in the normoxic group(P＜0.01,P＜0.01,P＜0.001).Resveratrol increased the expression level of LC3B and ATG5 in the hypoxic group(P＜0.05,P＜0.01),but did not upregulate the expression of Beclin1(P＞0.05).Conclusion:Hypoxia may inhibite the autophagy level of spleen in mice.Resvera-trol derivatives increases the autophagy level of mice under hypoxia condition,but has no significant effect on Beclin1 expression.
