Static compressive stress accelerting autophagy of human ligament cells in three-dimensional culture
10.3969/j.issn.1001-3733.2025.02.008
- VernacularTitle:静态压应力促进三维培养下的人牙周膜细胞自噬
- Author:
Shiyang WU
1
;
Xueping KANG
1
;
Rui ZOU
1
;
Zhen CHAI
1
;
Lingling JI
1
Author Information
1. 710004,西安交通大学口腔医院,陕西省颅颌面精准医学研究重点实验室,陕西省牙颌疾病临床研究中心
- Publication Type:Journal Article
- Keywords:
Autophagy;
Collagen-alginate hydrogels;
Human periodontal ligament cells;
Static compressive stress
- From:
Journal of Practical Stomatology
2025;41(2):189-192
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a three-dimensional culture and stress-loaded model of human periodontal ligament cells(hP-DLCs)in vitro and to determine the effect of static pressure on autophagy.Methods:Periodontal tissue was scraped from fresh ex-tracted tooth surfaces for culture and hPDLCs were obtained.Type Ⅰ rat tail collagen was extracted to prepare collagen-sodium algi-nate composite hydrogel,in which third-generation hPDLCs were embedded.After 72 h of culture,gene expression of autophagy-related proteins LC3,Atg-5 and Beclin-1 was detected by quantitative real-time polymerase chain reaction(qRT-PCR),and the expression of Atg-5,Beclin-1 and LC3 was detected by Western blotting.Results:Cells grew well in collagen-alginate hydrogels.After 15 min of compressive stress loading,the level of autophagy of hPDLCs increased significantly and then gradually decreased to the baseline level.Conclusion:Collagen-alginate hydrogel has good biocompatibility and safety,and can be used as an excellent scaffold for three-dimensional cell culture in vitro.Static compressive stress can accelevate autophagy,and this acceleration is rapid and short-lived.
