Regulation of methyltransferase METTL3 on radiotherapy sensitivity of oral squamous cell carcinoma cells
10.3969/j.issn.1001-3733.2025.02.011
- VernacularTitle:甲基转移酶METTL3对口腔鳞癌细胞放疗敏感性的调控作用研究
- Author:
Qingzhe MENG
1
;
Junhong HUANG
;
Xinjie YANG
;
Huan LI
;
Zihui YANG
;
Jun WANG
;
Yahui LI
;
Rong LIU
;
Jianhua WEI
Author Information
1. 154000,佳木斯大学附属口腔医学院·附属口腔医院黑龙江省口腔生物医学材料及临床应用重点实验室
- Publication Type:Journal Article
- Keywords:
METTL3;
Methylation;
Radiosensitivity;
Oral squamous cell carcinoma
- From:
Journal of Practical Stomatology
2025;41(2):206-213
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the influence of methyltransferases like 3(METTL3)on the radiosensitivity of oral squamous cell carcinoma cells(OSCCs).Methods:The apoptosis level of OSCCs CAL27,SCC9 and SCC15 treated with X-ray radiation doses of 2,4 and 8 Gy respectively was compared by flow cytometry,the expression of methylated gene RNA and protein in the cells were examined by qRT-PCR and Western blot.m6A in the cells was quantified by LC/LC-MS method.qRT-PCR and Western blot were used to investigate the expression of methylated gene RNA and protein in the cells.Flow cytometry was used to examine the cell apoptosis level of CAL27 and SCC15 cells treated with METTL3 overexpression and knockdown respectively.The clone forma-tion of CAL27 and SCC15 cells after knockdown and overexpression of target genes followed by radiation treatment was observed by clonogenic assays.Results:The apoptosis rate of all the cell lines increased with the increase dose of radiation at each dose,CAL27 cells showed the highest and SCC15 showed the lowerst apoptosis rate.The RNA and protein expression levels of METTL3 in CAL27 were significantly lower than those of SCC15.m6A quantification showed that the methylation modification in CAL27 cells was lower than that in SCC15.The expression of METTL3 was increased in CAL27 and SCC15 cells after radiation treatment.Knockdown of METTL3 increaced the apoptosis rate and decreased the clonogenesity,overession of METTL3 the decreaced the ap optosis rate and increased the clonogenecity of the cells.Conclusion:Regulation of METTL3 can affect the radiotherapy sensitivity of OSCCs,METTL3 may become a new target for radiosensitization of OSCCs.
