Identification of porcine reproductive and respiratory syndrome infection in pig herds from Henan Province
10.16303/j.cnki.1005-4545.2025.03.05
- VernacularTitle:从河南省猪群中鉴定出PRRSV1病毒感染
- Author:
Xingang WANG
1
;
Zhanda GUO
;
Jimei DU
;
Xingyu JI
;
Xianfeng ZHANG
;
Lu CHEN
;
Yan-hui WANG
;
Chuanqing WANG
Author Information
1. 商丘美兰生物工程有限公司,河南商丘 476200
- Publication Type:Journal Article
- Keywords:
PRRSV;
PRRSV1;
ORF5 gene;
whole gene sequencing;
genetic evolution analysis
- From:
Chinese Journal of Veterinary Science
2025;45(3):427-435
- CountryChina
- Language:Chinese
-
Abstract:
In order to understand the epidemic situation of European porcine reproductive and re-spiratory syndrome virus 1(PRRSV1)in Henan Province,molecular epidemiological investigation and virus identification were conducted on clinical specimens from some large-scale pig farms in Henan Province.RT-PCR detection and sequencing were carried out using specific primers for PRRSVI ORF5 gene.As a result,eight PRRSV1 positive samples were detected with six ORF5 gene sequences and one full gene sequence obtained.A strain of PRRSVI named as HENZMD-10 was successfully isolated using PAM cells.Based on the genetic variation analysis,the six ORF5 genes obtained were all PRRSVI.The isolated strains have relatively large ORF5 gene genetic vari-ation,belonging to different branches on the genetic evolution tree.Among them,HENJZ-11,HENJY-7,and HENJY-8 were relatively close in genetic evolution and belonged to the same branch.The genome length of HENZMD-10 isolate was 15 071 bp.Alignment analysis showed that HENZMD-10 strain shared an 89.1％of nucleotide sequence with the LV strain;62.1％and 61.5％with PRRSV2 ATCC-VR2332 strain and the American NADC30 strain,respectively.The nucleotide similarity of HENZMD-10 strain with the domestic JXA1 strain was 61.6％.The phylo-genetic analysis using complete genome sequence showed that HENZMD-10 strain was relatively close to the domestic isolated PRRSV1 NVDC-NM1-2011,LNEU12 and FJEU13 strains.The suc-cessful isolation of one PRRSV1 strain in Henan Province provides the basis for understanding the epidemic dynamics and prevention and control of PRRSV1 in China.
