The effect of perineural invasion on immune cell infiltration in pancreatic ductal adenocarcinoma by CIBERSORT
10.3781/j.issn.1000-7431.2024.2204-0254
- VernacularTitle:CIBERSORT分析胰腺导管腺癌神经侵袭对免疫浸润的影响
- Author:
Dapeng XU
1
;
Hongfei YAO
;
Yichao LIN
;
Yu XU
;
Ding MA
;
Minwei YANG
;
Jianyu YANG
;
Rong HUA
;
Yongwei SUN
;
Junfeng ZHANG
Author Information
1. 上海交通大学医学院附属仁济医院胆胰外科,上海 200127
- Publication Type:Journal Article
- Keywords:
Pancreatic ductal adenocarcinoma;
Perineural invasion;
Immune infiltration;
CIBERSORT
- From:
Tumor
2024;44(5):500-509
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the effect of perineural invasion(PNI)on immune cell infiltration in pancreatic ductal adenocarcinoma(PDAC)by the CIBERSORT deconvolution algorithm.Methods:The pancreatic cancer patients from the dataset GSE102238 were re-evaluated for the severity of PNI.And then the high and low PNI subgroups were subjected to immune scoring and immune cell infiltration analysis using the ESTIMATE and CIBERSORT algorithms to find immune cell subgroups associated with PNI.Finally,the results were validated by tissue microarrays.Results:Twenty-five cases were selected from 50 pancreatic cancer specimens for PNI reassessment and then divided into two high and low groups.Compared to the low PNI group,specimens from patients in the high group showed significantly less CD8+T-cell infiltration(P<0.05)and significantly more resting memory CD4+T-cells and M0 macrophages(P<0.05).Significantly reduced CD8+T cells(P<0.01)and slightly increased CD4+T cells(P<0.05)were confirmed in the patients with the high PNI using tissue microarrays.Meanwhile,macrophages significantly increased in the high PNI group(P<0.001).Conclusion:High PNI in PDAC inhibits infiltration of CD8+T cells which promote the infiltration of macrophages.
