Single-cell RNA sequencing and Mendelian randomization to analyze regulatory role of key genes in IgA nephropathy
10.3969/j.issn.1673-9701.2024.35.002
- VernacularTitle:单细胞RNA测序和孟德尔随机化分析IgA肾病关键基因的调控作用
- Author:
Minhua QIU
1
;
Peng JIANG
;
Jibing CHEN
;
Ting ZHANG
;
Hongjun GAO
Author Information
1. 广西中医药大学研究生院,广西南宁 530001;广西中医药大学附属瑞康医院器官移植科,广西南宁 530011
- Publication Type:Journal Article
- Keywords:
Single-cell RNA sequencing;
Mendelian randomization;
IgA nephropathy;
Key genes;
Chronic kidney disease
- From:
China Modern Doctor
2024;62(35):6-10,16
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore gene expression of key cell types in IgA nephropathy(IgAN)and regulatory role of key genes ADI1,BNIP3L and SERPINE2 in occurrence and development of IgAN.Methods The gene expression omnibus(GEO)and eQTLGen consortium database were used to perform Mendelian randomization(MR)and single-cell RNA sequencing(scRNA-seq).Key cell types of IgAN and causal relationship between ADI1,BNIP3L,SERPINE2 and IgAN were investigated.Expression profiles and screened out low expression genes were read through Seurat package,and used differential gene expression analysis to screen TOP100 highly expressed genes.In addition,were used sensitivity analysis and heterogeneity test were used to select significant results.Results ADI1(OR=0.946,95％CI:0.900-0.994,P=0.029),BNIP3L(OR=0.820,95％CI:0.707-0.950,P=0.008),SERPINE2(OR=0.958,95％CI:0.919-0.999,P=0.045)might be associated with a lower risk of IgAN.However,KLF6(OR=1.318,95％CI:1.036-1.678,P=0.025)was associated with high risk of IgAN.Conclusion Genetic characteristics of 18 key cell types including proximal renal tubule cells were revealed.ADI1,BNIP3L and SERPINE2 are closely related to the regulation of IgAN.
