Comined Antimicrobial Susceptibility Test of 12 Antibiotic Combinations Against Carbapenem-resistant Acinetobacter Baumannii
10.3969/j.issn.1671-7414.2025.02.012
- VernacularTitle:12种抗生素组合对耐碳青霉烯类鲍曼不动杆菌联合药敏试验
- Author:
Dongping WANG
1
;
Aimei SU
1
;
Xinyi HAN
1
Author Information
1. 青岛市第八人民医院检验科,山东 青岛 266100
- Publication Type:Journal Article
- Keywords:
carbapenem-resistant Acinetobacter baumannii;
checkerboard testing;
combined antimicrobial susceptibility test;
fractional inhibitory concentration index
- From:
Journal of Modern Laboratory Medicine
2025;40(2):64-69
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the in vitro susceptibility test result of colistin(COL)combined with tigecycline(TGC),cefoperazone/sulbactam(SCF),ampicillin/sulbactam(SAM),meropenem(MEM),amikacin(AK)and levofloxacin(LEV),TGC combined with MEM,SCF,SAM and AK,SCF combined with MEM,AK to carbapenem-resistant Acinetobacter baumannii(CRAB),and screen the effective anti-infective therapy.Methods 58 CRAB strains were isolated from clinic specimens from Qingdao Eighth People's Hospital from January 2023 to December 2023.The minimal inhibitory concentration(MIC)of antimicrobial agents against CRAB strains was determined by the micro broth dilution method.A combined antimicrobial susceptibility test was performed by the chessboard dilution method.The fractional inhibitory concentration(FIC)index was calculated to determine the combined effect.Results The synergistic rates of COL and SCF was 70.69%,and the sum of synergistic rates and additive rates was 96.55%.The synergistic rates of COL and TGC,COL and SAM,COL and MEM,COL and AK,COL and LEV,TGC and SCF,TGC and SAM,TGC and MEM,TGC and AK,SCF and MEM,SCF and AK were 34.48%,65.52%,51.72%,37.93%,22.42%,27.59%,27.59%,37.93%,41.38%,48.28%and 25.86%,and the sum of synergistic rates and additive rates were 77.58%,91.38%,84.48%,65.52%,39.66%,67.25%,62.07%,58.62%,55.17%,82.76%and 74.14%,respectively.The range,MIC50 and MIC90 of 12 combination regimens for CRAB strains were decreased to some extent compared with those of each single drug.No antagonism effect was observed for all the combinations.Conclusion The sum of synergy rate and addition rate of COL combined with SCF is the highest,and the sum of synergy rate and addition rate of COL combined with LEV is the lowest,which could provide reference for clinical experience medication.
