The Effect of MAGE-A3 Gene on the Biological Behavior and Drug Resistance of Ovarian Cancer
- VernacularTitle:MAGE-A3基因对卵巢癌的生物行为学及耐药性的影响
- Author:
Rumeng WANG
1
;
Ning ZHANG
;
Yanan JI
Author Information
1. 巴彦淖尔市医院中心实验室,内蒙古巴彦淖尔 015000
- Publication Type:Journal Article
- Keywords:
Ovarian cancer;
Melanoma-associated antigen A3;
Invasion;
Migration;
Apoptosis;
Drug resistance
- From:
Journal of Practical Obstetrics and Gynecology
2025;41(2):150-157
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of knockdown and overexpression of melanoma-associated antigen-A3(MAGE-A3)gene on the biological behavior and cisplatin resistance of ovarian cancer cells.Methods:The expression level of MAGE-A3 mRNA and protein in ovarian cancer cells was detected.The knockdown cell lines were constructed and divided into control group,no-load(NC)group and sh MAGE-A3 group.The overex-pression cell lines were divided into control group,no-load(NC)group and OE-MAGE-A3 group.The effects of knockdown and overexpression of MAGE-A3 gene on the proliferation,migration,invasion and apoptosis of tumor cells were compared.The expression of p53,Survivin,breast cancer susceptibility gene 1(BRCA1)and MAGE-A3 protein was detected.The high expression cell lines were divided into control group,cisplatin group,shMAGE-A3 group and cisplatin+shMAGE-A3 group.The low expression cell lines were divided into control group,cisplatin group,OE-MAGE-A3 group and cisplatin+OE-MAGE-A3 group.The cell proliferation,invasion ability of each group of cells,as well as their effects on the mRNA and protein expression level of each gene were compared.Results:The mRNA and protein expression of MAGE-A3 was high in SKOV3 cells and low in A2780 cells.Com-pared with the control group and NC group,the shMAGE-A3 group had lower OD450 value,migration rate and in-vasion numbers,higher apoptosis rate,lower expression of MAGE-A3 and Survivin protein,and higher expression of BRCA1 and p53 protein.The results of OE-MAGE-A3 group compared with control group and NC group were opposite to those of shMAGE-A3 group.Compared with the control group,cisplatin group and sh-MAGE-A3 group,the cisplatin+sh-MAGE-A3 group had lower OD450 value and invasion number higher levels of p53 and BRCA1 mRNA and protein expression,and lower levels of Survivin mRNA and protein.The OD450 value and in-vasion number of cells in cisplatin+OE-MAGE-A3 group were higher than those in cisplatin group,but lower than those in control group and OE-MAGE-A3 group.The mRNA and protein expression levels of p53 and BRCA1 were higher than those in the control group and the OE-MAGE-A3 group,but lower than those in the cisplatin group.The expression levels of Survivin mRNA and protein were lower than those in the control group and the OE-MAGE-A3 group,and higher than those in the cisplatin group.Conclusions:Knockdown of MAGE-A3 expression can effectively inhibit the proliferation,migration and invasion of ovarian cancer cells,promote apoptosis and en-hance cisplatin sensitivity.On the contrary,overexpression of MAGE-A3 increases cell proliferation,migration and invasion,and reduced apoptosis,thereby conferring cisplatin resistance.
