Effect of hyperbaric oxygen on the resistance of gastric cancer AGS cells to irinotecan and its regulatory mechanisms
10.3760/cma.j.cn311847-20210610-00181
- VernacularTitle:高压氧处理胃癌AGS细胞对伊立替康耐药性的影响及其调控机制
- Author:
Yanying XIE
1
;
Ziheng ZHENG
1
;
Lulu YANG
1
;
Chunjin XU
1
Author Information
1. 商丘市第一人民医院消化内科，商丘 476100
- Publication Type:Journal Article
- Keywords:
Hyperbaric oxygen;
Irinotecan;
Gastric cancer;
AGS cells
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2022;29(2):225-229
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of hyperbaric oxygen（HBO）on the resistance of gastric cancer AGS cells to irinotecan（ITC）and its related regulatory mechanisms.Methods:Gastric cancer AGS cells were cultured in vitro，and the cells were divided into four groups according to the experimental design：control group，ITC group，HBO group，and HBO + ITC group. The effect of ITC combined with HBO on the proliferation rate of gastric cancer cells was detected by Cell Counting Kit-8（CCK-8）；the changes of apoptosis rate after ITC combined with HBO treatment of AGS cells were detected by flow cytometry；and the changes in the expression of apoptosis-related and drug resistance-related proteins in AGS cells were detected by Western blotting assay. Results:Compared with control group and HBO group，the proliferation of AGS cells in the HBO + ITC group was further inhibited，and its proliferation rate was significantly lower than those of the control group and HBO group（ P<0.01），and also lower than that of the ITC group（ P<0.05）. Compared with those in the control group and the HBO group，the apoptosis rate of AGS cells in the HBO+ITC group was significantly higher than those in the control group and the HBO group（ P<0.01），and also higher than that in the ITC group（ P < 0.05）. In the ITC group and HBO+ITC group，the expression of apoptosis-related and drug resistance-related proteins，including Bcl-2，caspase-9 and P-gp，were significantly decreased，while the expression of Bad，caspase-3 and PARP were significantly increased，and the differences were statistically significant（ P<0.05 or P<0.01）. Compared with the control group and HBO group，p-JNK expression was increased and STAT3 expression was decreased in the ITC group（ P<0.01）；compared with the ITC group，p-JNK expression was increased and STAT3 expression was decreased in the HBO+ITC group（ P<0.05 or P<0.01）. Conclusion:HBO combined with ITC can effectively inhibit the proliferation of gastric cancer AGS cells，affect the apoptosis of AGS cells by regulating the JNK/STAT3 signaling pathway，and improve the cells’ drug resistance to a certain extent.
