Preliminary examination of the genetic characteristics and antimicrobial resistance genes of Salmonella Alachua
10.3969/j.issn.1002-2694.2025.00.021
- VernacularTitle:阿拉丘沙门菌的遗传特征及耐药基因初步研究
- Author:
Yue LIU
1
;
Xue-bin XU
;
Yi HU
;
Qi-fang GU
;
Cheng LIU
;
Zhen-an YUAN
;
Min CHEN
Author Information
1. 上海市疾病预防控制中心病原生物检定所,上海 200336
- Publication Type:Journal Article
- Keywords:
Salmonella Alachua;
blaKPC-2;
fosA7;
phylogenetic evolution
- From:
Chinese Journal of Zoonoses
2025;41(2):164-170
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed at preliminarily investigating the genetic and antimicrobial resistance characteristics of Salmonella Alachua isolates through whole-genome analyses.Five Salmonella Alachua isolates from various sources(both hu-man and non-human)were collected and identified.Phenotype and serotype verification,antimicrobial susceptibility testing,and whole-genome sequencing were performed.Virulence genes,antimicrobial resistance genes,and plasmid replicons were predicted according to globally available Salmonella Alachua genomic data.A phylogenetic tree was constructed to explore the genetic background.The first report of Salmonella Alachua in China emerged in Shanghai in 2015,and patients presented pri-marily with diarrhea.The isolates have been found predominantly in the eastern and southern coastal regions.Among the five i-solates analyzed,four belonged to sequence type(ST)2061,and one belonged to ST1298.All isolates were susceptible to most commonly used clinical antibiotics.Whole-genome analyses revealed that two ST2061 strains carried the blaKPC-2 gene,and one ST1298 strain carried the fosA7 gene.Phylogenetic analysis of global Salmonella Alachua populations indicated that the ST2061 clone belonged to the C1 clade,which was closely related to strains from the UK,whereas the ST1298 clone was found in the C4 clade,a globally disseminated fosA 7-positive lineage.This study provides initial insights into the genetic and antimi-crobial resistance characteristics of Salmonella Alachua in China,highlighting the presence of strains carrying blaKPC-2 and fo-sA7 genes.These findings may provide a reference for future large-scale molecular epidemiological surveillance and source-trac-ing efforts,and they underscore the importance of enhanced resistance monitoring for Salmonella Alachua.
