Cell-free DNA in blastocyst fluid for screening of embryo aneuploidy
10.3969/j.issn.1673-9701.2025.29.002
- VernacularTitle:囊胚腔液游离DNA用于胚胎非整倍体的筛查研究
- Author:
Huanli YANG
1
;
Yiyang ZHU
1
;
Wenhao LU
1
;
Jiaojiao CAI
1
Author Information
1. 浙江省台州医院生殖中心,浙江台州 317000
- Publication Type:Journal Article
- Keywords:
Blastocyst fluid;
Cell-free DNA;
Embryo aneuploidy;
Screening
- From:
China Modern Doctor
2025;63(29):5-9
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the feasibility of using cell-free DNA(cfDNA)in blastocyst fluid for preimplantation embryo aneuploidy.Methods A total of 17 patients undergoing assisted reproductive technology at Taizhou Hospital of Zhejiang from 2023 to 2024.A total of 38 discarded blastocysts were collected.Whole-genome amplification and next-generation sequencing were performed on both blastocyst fluid cfDNA and trophoblast cells.Using the trophoblast cells as reference,the amplification success rates of blastocyst fluid cfDNA were compared.The consistency,specificity,and sensitivity of the detection results were evaluated.Results The success rate of cfDNA amplification in the blastocyst fluid was 78.95％,and that of the trophoblast cells was 90.61％,with a consistency of 57.1％.The sensitivity of cfDNA amplification in the blastocyst fluid was 100％,the specificity was 66.7％,the positive likelihood ratio was 3,and the negative likelihood ratio was 0.Conclusion Blastocyst fluid cfDNA screening can be used as a screening method to exclude chromosomal embryos aneuploidy and can be used for preimplantation embryo selection.
