The effects of quetiapine on myelin morphology and expression of myelin basic protein in the corpus callosum of C57BL/6 mice with chronic alcohol exposure
10.3760/cma.j.issn.1006-7884.2015.03.017
- VernacularTitle:喹硫平对慢性酒精暴露C57BL/6小鼠胼胝体髓鞘形态和髓鞘碱性蛋白表达的影响
- Author:
Meng LIU
1
;
Xinjuan LI
;
Shuang LI
;
Libin ZHANG
;
Jinhong HAN
;
Ying ZHAO
;
Ruiling ZHANG
Author Information
1. 453002,新乡医学院第二附属医院精神科
- Publication Type:Journal Article
- Keywords:
Quetiapine;
Alcohol;
Myelin damage;
Myelin basic protein
- From:
Chinese Journal of Psychiatry
2015;48(3):182-187
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of quetiapine on cognitive function,myelin morphology and expression of myelin basic protein in the corpus callosum of C57BL/6 mice with chronic alcohol exposure.Methods According to random number table,120 male C57BL/6 mice were divided into 6 groups,i.e.,control group,quetiapine control group (10 mg· kg-1· d-1quetiapine),alcohol group,5 mg· kg-1· d1quetiapine plus alcohol group,10 mg· kg-1· d-1 quetiapine plus alcohol group and 15 mg·kg-1· d-1quetiapine plus alcohol group,with 20 mice in each group.Mice in control group and quetiapine control group were maintained with water and solid diet for 20 weeks.Mice in alcohol group and quetiapine plus alcohol groups were maintained with water containing volume fraction of 10％ alcohol and solid diet for 20 weeks.Drug with different doses was dissolved in mice drinking for intervention beginning at the 6th week and continuing to the end of the experiment.Morris water maze experiment was used to test the cognitive function.Luxol fast blue (LFB) staining and electron microscopy were used to observe morphology of corpus callosum.The expression of myelin basic protein in the corpus callosum was evaluated with immunofluorescence.Results (1)Morris water maze test showed that the cognition of mice in each group was different (F=4.702,P=0.002).The latency of mice in alcohol group ((22.8 ±5.5) s) was significantly longer compared with that of the control group((1 1.5± 7.1) s,t=3.546,P＜0.05).The latency of 5 mg· kg1 · d-1and 10 mg· kg-1 · d-1quetiapine plus alcohol groups ((19.1±8.8) s,(20.1±8.3) s) were not significantly different compared with that of the alcohol group (t=0.989,0.748,both P＞0.05).The latency of 15 mg· kg-1· d-1quetiapine plus alcohol group((11.5 ± 5.8) s) was significantly shorter compared with that of the alcohol group (t=3.998,P＜0.05).The latency of quetiapine control group ((10.6±5.5) s) was not significantly different compared with that of the control group (t=0.276,P＞0.05).(2)LFB staining and electron microscope results showed severe myelin damage in the corpus callosum of mice in alcohol group,while the integrity of the myelin sheath in 10 mg· kg-1· d 1and 15 mg·kg-1· d-1quetiapine plus alcohol groups improved significantly compared with the alcohol group.The integrity of the myelin sheath in quetiapine control group was not significantly different compared with that of the control group.(3) Inmunofluorescence results showed that the expression of myelin basic protein of each group was different (F=28.971,P＜0.01).The expression of myelin basic protein in alcohol group (0.038±0.005) was significantly decreased compared to the control group (0.062±0.005,t=8.628,P＜0.05).The expression of myelin basic protein in 5 mg·kg-1· d-1 quetiapine plus alcohol group (0.043±0.003)was not significantly different compared with the alcohol group (t=2.081,P＞0.05).The expression of myelin basic protein of 10 mg·kg-1· d-1 and 15 mg·kg-1· d-1 quetiapine plus alcohol groups (0.047±0.04,0.058±0.006) were significantly increased compared with the alcohol group (t=3.301,6.645,both P＜0.05).The expression of myelin basic protein in quetiapine control group (0.061±0.005)was not significantly different compared with the control group (t=0.041,1.137,both P＞0.05).Conclusion Chronic alcohol exposure could induce cognition impairment and white matter myelin danage in mice.Quetiapine treatment may prevent the brain white matter damage due to chronic alcohol exposure in mice.
