Phospho cAMP response element-binding protein 1 expression level and the ultrastructure of neuron and synapse in hippocampus of depression rats
10.3760/cma.j.issn.1006-7884.2015.02.009
- VernacularTitle:抑郁症模型大鼠海马区磷酸化环磷腺苷反应元件结合蛋白1表达水平及神经元和突触超微结构的研究
- Author:
Maolin HU
1
;
Xiaofen ZONG
;
Wei LIANG
;
Hongmei ZHANG
;
Zuoming CHEN
Author Information
1. 中南大学湘雅二医院精神卫生研究所
- Publication Type:Journal Article
- Keywords:
Depressive disorder;
Cyclic AMP response element-binding protein;
Neuronal plasticity;
Hippocampus
- From:
Chinese Journal of Psychiatry
2015;48(2):92-97
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the alterations of ultrastructure of neuron and synapse in rat hippocampus induced by the expression changes of phospho cAMP response element-binding protein 1 (p-CREB1),and the effect of venlafaxine on the expression of p-CREB1 and relative neuronal and synaptic plasticity.Methods Fifty-four rats were randomly divided into 4 groups:normal group (n =12),depression model matched group (n =14),saline group (n =14),and venlafaxine medication group (n =14).Sucrose water consumption test and open field test were performed to observe the behavior of animals.To investigate the learning and memory ability,rats were tested by Morris water maze.The expression of p-CREB1 protein was detected by immunohistochemistry and the CREB1 mRNA was measured by RT-PCR.The ultrastructure of neuron and synapse were observed with electron microscope.Correlation analysis was used to measure the associations among the number of p-CREB1 positive cells,the parameters of Morris water maze and synaptic morphology.Results (1) The sucrose consumption in depression model matched group (7.4 ± 1.0) ml/100 g and saline group (7.5 ± 1.0) ml/100 g were significantly less than that in normal group (9.6 ± 0.3) ml/100 g and medication group (9.4 ± 0.8) ml/100 g,(F =17.851,P ＜ 0.01).Morris water maze showed that the escape latent period in depression model matched group (61.1 ± 10.5) s and saline group (59.0 ± 10.6) s were more than that in normal group (29.8 ± 10.1) s and medication group ((35.0 ± 8.5) s;F =30.559,P ＜ 0.01),which demonstrated the learning and memory ability of depression rats were decreased.(2) The positive cell number of p-CREB1 in the hippocampus of depression model matched group (21.07 ±5.99) and saline group (24.57 ±6.97) were lower than that in normal group (29.70 ± 6.21) and medication group (41.50 ± 11.95;F =16.497,P ＜ 0.01).(3) The CREB1 mRNA expression in the hippocampus of depression model matched group (0.58 ± 0.47) and saline group (0.45 ± 0.24) were less than that in normal group (1.03 ± 0.89) and medication group (1.10 ± 0.45;F =6.669,P ＜ 0.01).(4) There were pathologic alterations in the ultrastructure of neurons and synapses in the hippocampus of depression rats,which were improved by pharmacological intervention.(5) The number of p-CREB1 positive cells was related to the parameters of Morris water maze and synaptic morphology.Conclusion p-CREB1 expression is correlated with neuronal and synaptic plasticity,and venlafaxine may be effective through influencing the expression of p-CREB1 and neuronal and synaptic plasticity.
