The efficacy of minocycline on behavioral deficits and microglial activation induced by intrahippocampal injection of lipopolysaccharide
10.3760/cma.j.issn.1006-7884.2015.01.007
- VernacularTitle:米诺环素对精神分裂症模型大鼠行为学异常及小胶质细胞活性的影响
- Author:
Furong ZHU
1
;
Jingping ZHAO
;
Yingjun ZHENG
;
Fang LIU
;
Jianjun OU
Author Information
1. 中南大学湘雅二医院精神卫生研究所,长沙,410011
- Publication Type:Journal Article
- Keywords:
Schizophrenia;
Minocycline;
Microglia;
Negative symptoms
- From:
Chinese Journal of Psychiatry
2015;48(1):27-31
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the efficacy of minocycline on behavioral deficits and microglial activation in the microglial activation model of schizophrenia (SZ).Methods Neonatal intrahippocampal injection of lipopolysaccharide (LPS,10 g/L) or saline (0.3 μl) in rats was performed at postnatal day 7.The rats in two groups were randomly assigned using random number table to be given risperidone (0.5 mg/kg),minocycline(40 mg/kg),combination of both of them or saline (10 ml/kg) at postnatal day 42 for two weeks.Then behavioral changes (social behavior using open field box and prepulse inhibition (PPI) using startle reflex test system) and cytokines (IL-1 β and TNF-α) in hippocampus were examined.Results The adult rats in LPS-injected group showed obvious decreased social behavior(social number:10.50 ±1.73 ;social time:(18.67 ± 1.96) s;all P ＜ 0.01),decreased PPI (all P ＜ 0.01),increased IL-1 β/β-actin and TNF-α/β-actin(all P ＜ 0.01),compared to saline group(social number:16.50 ± 1.61 ; social time:(45.28 ± 4.67) s).Compared to LPS-group rats,the rats in risperidone-group,minocycline-group,risperidone combination with minocycline-group showed obvious increased social behavior and PPI,there were significantly decreased IL-1β/β-actin and TNF-α/β-actin in minocycline-group and risperidone combination with minocycline-group (all P ＜ 0.01) while not in risperidone-group.Conclusions Minocycline could significantly rescue SZ-like behavioral deficits and inhibit the increased cytokines in the microglial activation model of SZ,and the treatment mechanism may be related with the inhibition of microglial activation.
