Establishment of an animal model with controllable osteoradionecrosis of the jaws with bone defect range
10.3969/j.issn.1005-4847.2025.02.007
- VernacularTitle:骨缺损范围可控颌骨放射性骨坏死动物模型的建立
- Author:
Jialing TIAN
1
;
Bizhou ZHAO
1
Author Information
1. 甘肃省中医院口腔颌面外科,兰州 730050
- Publication Type:Journal Article
- Keywords:
osteoradionecrosis of jaws(ORNJ);
bone defect;
controllable;
animal model
- From:
Acta Laboratorium Animalis Scientia Sinica
2025;33(2):225-231
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish,based on new diagnostic criteria for osteoradionecrosis of the jaws(ORNJ),an animal model with a controllable bone defect range and early and mild ORNJ characteristics.Methods A method involving single high-dose irradiation with an electron linear accelerator was applied.According to the irradiation dose,the animals were divided into a group(control group);b group(12 Gy group);and c group(14 Gy group).Six rabbits were randomly assigned to each group,b and c groups were uniformly selected for further radiotherapy of the right mandibular area.After 1 week,all animals were prepared with a standard bone defect at the right mandibular angle.After 4 weeks,the animals were euthanized,and general observations were conducted.CBCT scans and HU values of mandibular specimens,HE staining histological observations,Trap staining histology,and osteoclast counts were compared.And the statistical analysis were carried out.Results 4 weeks after the bone defect was created,the soft tissue in the surgical area of b and c groups showed mild swelling,purple skin,and erosion and ulceration of the oral mucosa,with c group showing the most significant changes.CBCT examination showed that the cortical bone of the irradiated areas in b and c groups was rough,while that of c group had poor continuity.No significant changes were observed in the extent of the bone defect in any group,b and c groups had significantly decreased HU values compared with a group.HE staining and histological observation suggested that the bone continuity of b and c groups had deteriorated;there was a large amount of fibrotic tissue proliferation,an increase in blank bone pits without cells,and an infiltration of inflammatory cells.Trap staining revealed a significant increase in osteoclasts in c group compared with a group.Conclusion By using an electron linear accelerator,a single dose of 14 Gy irradiation was applied to the mandibular angle area to create bone defects with consistent specifications in rabbits.After 4 weeks,an animal model that met the early diagnostic criteria of ORNJ and had controllable consistency in the range and degree of bone defects was established.
