Exploration on the Mechanism of Astragali Complanati Semen in Preventing and Treating Hyperlipidemia Based on Network Pharmacology and Experimental Validation
10.19879/j.cnki.1005-5304.202505401
- VernacularTitle:基于网络药理学和实验验证探讨沙苑子防治高脂血症作用机制
- Author:
Juanjuan TAN
1
;
Wenqi ZHOU
;
Guorong DENG
;
Li CHENG
;
Fang GUAN
;
Qiong YU
;
Hongyi YANG
;
Feng HUANG
;
Yuanwang YU
;
Haifang WANG
;
Ruihua YUAN
Author Information
1. 陕西中医药大学整合医学研究院,陕西 咸阳 712046;陕西省中西医结合心血管病防治重点实验室,陕西 咸阳 712046
- Publication Type:Journal Article
- Keywords:
hyperlipidemia;
Astragali Complanati Semen;
network pharmacology;
molecular docking;
experimental validation
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2025;32(11):40-47
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of Astragali Complanati Semen in the prevention and treatment of hyperlipidemia;To provide theoretical basis for its clinical application.Methods The active components of Astragali Complanati Semen were retrieved and screened through TCMSP,TCMID and TDT databases to obtain the action targets of the active components.Hyperlipidemia targets were obtained through GeneCards,DisGeNET,and TTD databases,and the drug active component targets were intersected with hyperlipidemia targets.Cytoscape 3.9.1 software and STRING database were used to construct active component-target network and protein-protein interaction network,screening for major active components and core targets.GO and KEGG pathway enrichment analysis was performed using the DAVID database,and the CB-Dock platform was used for molecular docking.HepG2 cells were induced to construct a high-fat cell model using oleic acid and palmitic acid,and intervened with Astragali Complanati Semen freeze-dried powder solution.The mRNA expression of the core target was detected by RT-qPCR.Results A total of 10 active components of Astragali Complanati Semen and 67 potential action targets of hyperlipidemia were identified,involving signaling pathways such as AGE-RAGE,lipid metabolism,HIF-1,etc.Experimental results showed that intervention with Astragali Complanati Semen could reduce lipid accumulation in the high-lipid cell model,with an optimal intervention concentration of 500 μg/mL;RT-qPCR revealed significant down-regulation of TNFα,IL6,AKT1,PPARG,and other genes after intervention with Astragali Complanati Semen.Conclusion Astragali Complanati Semen exerts lipid-regulating effects through multiple targets and pathways,providing a basis for its application in the prevention and treatment of hyperlipidemia.
