Molecular genotypes and drug resistance characteristics of carbapenem-resistant Klebsiella pneumoniae isolates from children and adults in Kunming area
10.11816/cn.ni.2025-241177
- VernacularTitle:昆明地区儿童及成人碳青霉烯类耐药肺炎克雷伯菌分离株分子分型及耐药特征
- Author:
Wenting WANG
1
;
Jing QIAN
;
Rui ZHENG
;
Hanyu LIU
;
Tingyi DU
;
Li JIANG
Author Information
1. 昆明市儿童医院昆明医科大学附属儿童医院检验科,云南 昆明 650103
- Publication Type:Journal Article
- Keywords:
Klebsiella pneumoniae;
Molecular genotype;
Carbapenem resistance;
Child;
Adult;
Kunming;
Drug resistance gene
- From:
Chinese Journal of Nosocomiology
2025;35(9):1293-1298
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To analyze the homology and drug resistance characteristics of clinical carbapenem-resistant Klebsiella pneumoniae(CRKP)isolates from the children and adults in Kunming area so as to provide guidance for prevention and control of CRKP strains.METHODS Totally 41 strains of clinical CRKP isolates were collected from the children in 4 hospitals of Kunming area in 2023,and 54 strains were collected from the adults.The ho-mology of the strains was analyzed by means of repetitive extragenic palindromic-polymerase chain reaction(REP-PCR);the phenotypes of carbapenemases and the genes in the strains were detected.RESULTS The 95 strains of CRKP were divided into 30 genotypes.There was clustering of genotypes in some strains.The type A enzyme was most common among the strains from the adults(47 strains,87.04％),while the type A enzyme(18 strains,43.90％)and the type B enzyme(23 strains,56.10％)were both detected in the strains from the children.A-mong the strains producing type A enzyme,the strains carrying with KPC gene were both dominant among the strains isolated from the adults(44 strains,81.48％)and the strains isolated from the children(18 strains,43.90％).Among the strains producing type B enzyme,the strains carrying with NDM gene were dominant a-mong the strains from the children(22 strains,53.66％).CONCLUSIONS There is colonization and inter-hospital transmission of CRKP strains in the hospitals of Kunming area.It is necessary for the clinical departments of the children's hospitals to strengthen the active screening of the transferring children and carefully carry out the cleaning and disinfection of nosocomial environment so as to reduce the probability of the CRKP infection among the children.Mean-while,the clinicians of the pediatrics department should comprehensively take all the factors into accounts such as the type of enzyme,formulate the appropriate medication regimens and raise the cure rate of the children with CRKP infection.
