Ameliorative effects of Compound Fufangteng Mixture on cyclophosphamide-induced immunosuppression in mice
10.3969/j.issn.1001-1528.2025.10.011
- VernacularTitle:复方扶芳藤合剂对环磷酰胺诱导小鼠免疫抑制的改善作用
- Author:
Li-na LIU
1
;
Yu-fang SHEN
;
Qin-qin WANG
;
Lin-yu XIAO
;
Jing-yu LIU
;
Jun-ni MO
;
Ren-yi-kun YUAN
;
Hong-wei GAO
;
Jian XIAO
Author Information
1. 广西中医药大学药学院,广西南宁 530200;中药壮瑶药创新药物教育部工程研究中心,广西南宁 530200
- Publication Type:Journal Article
- Keywords:
Compound Fufangteng Mixture;
cyclophosphamide;
immunosuppression;
T lymphocytes;
macrophages
- From:
Chinese Traditional Patent Medicine
2025;47(10):3249-3256
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the ameliorative effects of Compound Fufangteng Mixture(CFM)on cyclophosphamide(CTX)-induced immunosuppression in mice.METHODS Forty-eight male C57BL/6J mice were randomly divided into the blank control group,the model group,the levamisole hydrochloride group(40 mg/kg)and the low-dose,medium-dose and high-dose CFM groups(3.75,7.5,10 g/kg),with 8 mice in each group,and given respective intervention orally once daily for 14 days.On the 5th to 7th day of administration,with the blank control group given normal saline intraperitoneally,the other groups underwent intraperitoneal CTX injections(80 mg/kg).24 hours after the last administration,organ indices of thymus and spleen were calculated;splenic histopathological alterations were assessed by HE staining;serum levels of IL-2,IL-6 and IgG were quantified using ELISA;splenic CD4+,CD8+T lymphocytes,alongside CD86+and CD206+macrophages populations were analyzed by flow cytometry;and splenic expression of CD4,CD8 and F4/80 was evaluated by immunohistochemical staining.RESULTS In CTX-treated mice,CFM administration mitigated body weight loss;enhanced thymus weight and thymic index;ameliorated splenic immune cell populations,elevated serum levels of cytokines IL-2,IL-6 and IgG in serum;and upregulated splenic levels of CD45+CD3+T lymphocytes and F4/80+CD11b+macrophages,alongside increasing the expression of CD4,CD8 and F4/80 surface markers.CONCLUSION CFM alleviates CTX-induced immunosuppression state in mice by modulating immune cells,restoring immune function and enhancing anti-inflammatory and tissue repair capabilities.
