Placenta derived mesenchymal stem cells inhibit the activation of rat astrocytes via TGF-β/Smad pathway
10.16557/j.cnki.1000-7547.2025.02.008
- VernacularTitle:胎盘间充质干细胞通过TGF-β/Smad通路抑制LPS诱导的大鼠星形胶质细胞活化
- Author:
Ningmei LIU
1
;
Taojuan WU
1
;
Dongmei CHEN
1
;
Ting LIU
1
;
Xiaona MA
1
;
Haibin MA
1
;
Xueyun LIANG
1
Author Information
1. 宁夏医科大学总医院宁夏干细胞与再生医学重点实验室,银川 750001
- Publication Type:Journal Article
- Keywords:
placenta-derived mesenchymal stem cells(PMSCs);
lipopolysaccharides(LPS);
neuroinflammation;
TGF-β/Smad signaling pathway;
rat
- From:
Chinese Journal of Neuroanatomy
2025;41(2):187-193
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects and its related mechanism of placenta-derived mesenchymal stem cells(PMSCs)on the lipopolysaccharides(LPS)damaged astrocytes.Methods:Primary astrocytes were isolated from the cerebral cortex of neonatal rats.The expression of glial fibrillary acidic protein(GFAP)was identified using immu-nofluorescence staining to evaluate the purity of the primary astrocytes.PMSCs were cocultured with LPS-treated astro-cytes.The expression levels of factors related to inflammation including interleukin-1β(IL-1β),inducible nitric oxide synthase(iNOS),tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),arginase-1(Arg-1),S100 calcium-bind-ing protein A10(S100A10),and TGF-β/Smad signaling pathway-related proteins such as transforming growth factor beta 1(TGF-β1),transforming growth factor beta type I receptor(TβRⅠ),transforming growth factor beta type II re-ceptor(TβRⅡ),phospho-Smad2 and phospho-Smad3(p-Smad2,p-Smad3)in astrocytes from each group were detec-ted using real time RT-PCR or Western blot techniques.Results:Astrocytes at the third passage exhibited an 80%pos-itivity rate for GFAP.After treated with 10 μg/ml LPS,the astrocytes expression levels of pro-inflammatory factors IL-1β,iNOS,IL-6,and TNF-α were significantly increased(P<0.05),while their expression levels of the anti-inflam-matory factors of Arg-1 and S100A10 were significantly decreased(P<0.05).Meanwhile,their expression levels of TGF-β/Smad signaling pathway related proteins of TGF-β1,TβRⅠ,TβRⅡ,p-Smad2 and Smad3 were increased(P<0.05).After the LPS damaged astrocytes were cocultured with PMSCs,their expression levels of pro-inflammatory factors IL-1β,iNOS,IL-6,and TNF-α were significantly decreased(P<0.05),while their expression levels of the anti-inflammatory factors of Arg-1 and S100A10 were significantly increased(P<0.05).Also,their expression levels of TGF-β/Smad signaling pathway related proteins of TGF-β1,TβRⅠ,TβRⅡ,p-Smad2,and Smad3 were decreased(P<0.05).Conclusion:PMSCs may inhibit the activation of A1 astrocytes through the TGF-β/Smad signaling path-way,by which reducing the astrocytic activation.
