Neuroactive substances gene expression changes in spinal trigeminal nucleus caudalis nucleus and trigeminal ganglia of rats by the exposure of repeated high +Gz
10.3760/cma.j.issn.1007-6239.2016.01.012
- VernacularTitle:重复高+Gz暴露后大鼠三叉神经脊束核尾侧亚核与三叉神经节内神经活性物质基因表达
- Author:
Xin CHEN
1
;
Yan FENG
;
Yin YIN
;
Junkai CAO
;
Hongshi LI
Author Information
1. 空军总医院口腔科, 北京,100142
- Publication Type:Journal Article
- Keywords:
Acceleration;
Hypergravity;
Rats;
Substance P;
Receptors,N-methyl-D-aspartate;
Trigeminal ganglion;
Trigeminal caudal nucleus;
Gene expression;
Sodium channels
- From:
Chinese Journal of Aerospace Medicine
2016;27(1):55-59
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the mRNA expression of substance P (SP),N-methyl-D-aspartate receptor antagonists (NMDAR) in rat's spinal trigeminal nucleus caudalis (SpVc) and preprotachykinin A (PPTA),β-calcitonin-gene-related peptide (β-CGRP),sodium channel (PN3),and to investigate the cause and mechanism of repeated high + Gz induced temporomandibular joint pain.Methods Thirty-six male SD rats (weight 200-300 g) were randomly divided into control,+5 Gz and +10 Gz groups.Rats in each group were respectively fixed at the end of animal centrifuge arm with prone position.Head was towards to the rotating center.The radius of the centrifuge is 1 m.+Gz pulses were applied on rats with 1 G/s onset rate for 30 s plateau duration.The centrifuge tests were done 5 times a day with 60 s intervals,4 days a week for 3 weeks.The +5 Gz and +10 Gz group was respectively exposed to + 5 Gz and +10 Gz peak.The control group was only fixed on centrifuge along with the test schedule but without centrifuge running.RNA was collected from TG and SpVc of rats.Fluorescent quantitative RT-PCR method was used to detect mRNA of SP,NMDAR,PPTA,β-CGRP,PN3 and NaN mRNA expression.Results ① Repeated high acceleration (+ Gz) exposure caused PPTA,β-CGRP,PN3 and NaN gene expression significantly increased in rats' TG.Differences among groups showed statistical significance (F=329-1 030,P＜ 0.01).The differences were in direct proportion to +Gz value.②The mRNA relative expressions of SP and MMDAR were significantly increased in rats' SpVc and the difference among groups was statistical significance (F=408,1 062,P＜ 0.01),and was in direct proportion to + Gz value.Conclusions Under repeated high acceleration (+ Gz),the biochemical characteristics of primary sensory neurons occur significant change.Nerve active substances play a role in the pain transmission caused by temporomandibular joint disease,which is related to the peripheral and central conduction mechanism of temporomandibular joint pain.
