Effects of the high + Gx exposure after simulated weightlessness on the expression of salivary alpha-amylase in parotid gland of monkey
10.3760/cma.j.issn.1007-6239.2015.04.007
- VernacularTitle:模拟失重后高+Gx暴露对猴腮腺组织唾液淀粉酶表达的影响
- Author:
Yang GUO
1
;
Zhongying NIU
;
Chuhua TANG
;
Jianzhong ZHANG
;
Yanhua ZHENG
Author Information
1. 解放军第三○六医院全军口腔疾病诊治中心, 北京,100101
- Publication Type:Journal Article
- Keywords:
Weightlessness simulation;
Hypergravity;
Macaca;
Parotid gland;
Salivary alpha-amylase
- From:
Chinese Journal of Aerospace Medicine
2015;26(4):272-277
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of the high + Gx exposure after 30-d simulated weightlessness on salivary alpha-amylase (sAA) expression in rhesus monkey parotid,in order to provide experimental reference for maintaining astronauts' oral health and health care.Methods According to the literature review and the actual situation of spaceflight,twenty-three healthy male rhesus monkeys were randomly divided into four groups:①control group (n=3);②30-d simulated weightlessness group (n=3);③+ 13 Gx/230 s group (n=3);④group D:high +Gx after 30 d simulated weightlessness exposure group (n =14).The group D was further divided into four subgroups according to the +Gx peak load:-+-11 Gx/270 s (group D1,n=3),+13 Gx/230 s (group D2,n=4),+15 Gx/200 s (group D3,n=4),+-13 Gx/230 s and 9 d's recovery (group D4,n=3).For building weightlessness model,the rhesus monkeys were put on bed with-10° head down tilt position to simulate the microgravity environment.The rhesus monkeys were fixed in Model-58 animal centrifuge exposing to high +Gx environment that was built by computer controlled para-hypergravity profile.Salivary glands were collected at the second day of experimental operation.The expression of a-amylase on protein and mRNA level was separately detected by Western blot and quantitative realtime polymerase chain reaction (qRT-PCR).All of the statistical analysis data were performed by using SPSS 17.0.Results On protein level,Western blot analysis showed that sAA expression in other experimental groups decreased significantly as compared with that in control group (F=80.381,P＜0.01).In groups of high +Gx exposure after simulated weightlessness,sAA expression showed a decreasing trend with the +Gx increase.However it rose again by 9-d recovery.On mRNA level,it displayed a conformably trend with that of protein level.Conclusions High +Gx exposure after 30-d simulated weightlessness can down-regulate sAA expression in parotid gland of rhesus monkey,and may cause the changes on components and functions of saliva.
