Analysis of toxic material basis of Dryopteris crassirhizoma by UPLC-ESI-MS/MS
10.3969/j.issn.1001-1528.2025.10.020
- VernacularTitle:UPLC-ESI-MS/MS法分析绵马贯众毒性物质基础
- Author:
Rong-hui ZHENG
1
;
Cui-jie WEI
;
Fei-fei XIE
;
Xin-ya WAN
;
Xiao-jie LIANG
;
Zhi-wen DUAN
;
Dong-mei SUN
;
Xiang-dong CEHN
Author Information
1. 广州中医药大学,广东 广州 510006;广东一方制药有限公司,广东佛山 528244
- Publication Type:Journal Article
- Keywords:
Dryopteris crassirhizoma Nakai;
toxic material basis;
UPLC-ESI-MS/MS;
heatmap cluster analysis;
partial least squares discriminant analysis
- From:
Chinese Traditional Patent Medicine
2025;47(10):3305-3314
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish a UPLC-ESI-MS/MS method for analyzing the toxic material basis of 95％ethanol cold soaked ultrasonic extract(EC),95％ethanol heated reflux extract(EH)and water decoction extract(WD)from Dryopteris crassirhizoma Nakai.METHODS The analysis was performed on a 25 ℃ thermostatic agilent ZORBAX RRHD StableBond C18 column(2.1 mm×150 mm,1.8 μm),with the mobile phase comprising of methanol-0.2％formic acid flowing at 0.30 mL/min,and heated electrospray ion source was adopted in positive and negative ion scanning.Compounds were identified by Compound Discover 3.3 software combined with the database and related literature,and the main differential components were screened by Heatmap cluster analysis and partial least squares discriminant analysis.RESULTS 72 compounds were identified(22 phloroglucinols,19 flavonoids,8 phenylpropanoids,6 terpenoids and 17 other components).The main toxic differential components were phloroglucinols such as flavaspidic acid AB,didemethylpseudoaspidin AA and filixic acid PBP,flavonoids such as(-)-epicatechin,(-)-epigallocatechin,cianidanol,and other compounds such as indole-3-carboxaldehyde.CONCLUSION This method can rapidly,effectively and comprehensively characterize the main chemical composition of D.crassirhizoma,and provide a reference for the study of its pharmacological mechanism.
