Sterilization effect verification of 3D-printed polylactic acid guide plate for orthopedic surgery
10.11816/cn.ni.2025-250556
- VernacularTitle:3D打印聚乳酸材质骨科手术导板灭菌效果验证
- Author:
Yanqiong LI
1
;
Aiwei YIN
;
Naihan CHEN
;
Xinghui HU
;
Haotian LUO
;
Wenpeng ZHAO
;
Gang WANG
Author Information
1. 云南省第一人民医院/昆明理工大学附属医院消毒供应中心,云南 昆明 650032
- Publication Type:Journal Article
- Keywords:
3D printing;
Polylactic acid;
Surgical guide plate;
Hydrogen peroxide plasma sterilization;
Ethylene oxide sterilization;
Pressure steam sterilization;
Sterilization effect
- From:
Chinese Journal of Nosocomiology
2025;35(20):3173-3176
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the effects of different sterilization methods on 3D-printed polylactic acid(PLA)guide plates for orthopedic surgery,and to analyze their physical properties,microbial contamination,structural performance and bio compatibility after sterilization.METHODS PLA guide plates for orthopedic surgery were prepared with 3D printing technology and divided into a blank group,a hydrogen peroxide plasma steriliza-tion group,an ethylene oxide sterilization group and a pressure steam sterilization group.Before and after sterili-zation,the changes in volume and weight of the surgical guide plates were measured,microbial detection was con-ducted,structural changes were observed with a laser scanning confocal microscope,and cell co-culture was con-ducted to evaluate biocompatibility,thereby the effects of different sterilization methods were assessed.RESULTS The three sterilization methods had no significant effect on the volume and weight of PLA surgical guide plates.Microbial detection showed that all three sterilization methods were effective in killing bacteria,and bacte-rial cultures were negative.Laser confocal scanning microscopy revealed that sterilization treatment caused certain changes to the microstructure of the surgical guide plates,but high-temperature sterilization had a more pro-nounced effect on the deformation of the guide plate edges.Cell co-culture results indicated that the surgical guide plates treated with the three sterilization methods exhibited acceptable cytotoxicity and had little effect on cell pro-liferation.CONCLUSIONS All three sterilization methods cause microstructural changes to the surgical guide plate.Among them,pressure steam sterilization significantly deforms the structure of the guide plate,directly af-fecting its precise positioning of mutual spatial distances,angular relationships and orientation during surgery.Al-though hydrogen peroxide plasma sterilization does not cause significant deformation,ethylene oxide sterilization has the least impact on material properties and structural stability while ensuring the sterilization effect of PLA surgical guide plates.
