The effects of subchronic aluminum exposure on nerve growth factor in hippocampus of offspring rats
10.16557/j.cnki.1000-7547.2025.02.007
- VernacularTitle:亚慢性铝暴露对子代大鼠海马神经生长因子表达的影响
- Author:
Yuhua YIN
1
;
Shuo YANG
;
Tian ZHANG
;
Hong GUO
;
Luwei ZHANG
;
Zan LIU
;
Xin LI
;
Lifeng ZHANG
Author Information
1. 沈阳医学院公共卫生学院,沈阳 110034;通辽职业学院护理系,通辽 028000
- Publication Type:Journal Article
- Keywords:
aluminum;
hippocampus;
learning and memory;
nerve growth factor(NGF);
offspring rat
- From:
Chinese Journal of Neuroanatomy
2025;41(2):181-186
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of aluminum exposure on learning and memory and the expression of nerve growth factor(NGF)in the hippocampus of offspring rats,and to investigate the mechanism by which aluminum impairs learning and memory function.Methods:Forty pregnant Wistar rats were randomly assigned to the Control group(Control),low-dose Al group(Al-L),medium-dose Al group(Al-M)and high-dose Al group(Al-H).The off-spring rats were fed with Al through breast milk from birth to weaning,while the rats in the control group were fed with distilled water.The maternal rats Al-L,Al-M and Al-H groups drank distilled water solution containing 2.0,4.0 and 8.0 g/L AlCl3,respectively.After weaning,the offspring rats in the aluminum exposure group drank distilled water so-lution containing 2.0,4.0 and 8.0 g/L AlCl3 by themselves until the 90th day after birth to establish the offspring rat model of subchronic aluminum exposure.After aluminum exposure,the shuttle box test was used to detect the learning and memory ability of offspring rats,and the body weight of offspring rats and hippocampus were weighed to evaluate the effect of aluminum exposure.The expression of nerve growth factor(NGF)protein in hippocampus of offspring rats was detected by Western blot,and the expression of NGF mRNA in hippocampus of offspring rats was detected by real time RT-PCR.Results:The body weight of offspring rats in Al-H group was significantly lower than that in the other three dose groups.In the shuttle box test,compared with the control group,the active avoidance response and passive avoid-ance response of the offspring rats in the aluminum exposure group showed a downward trend with the increase of alumi-num exposure dose,indicating that the learning and memory ability of the offspring rats in the aluminum exposure group was impaired.Compared with the control group,the NGF protein content and NGF mRNA expression in the hippocam-pus of offspring rats in the aluminum exposure group were significantly decreased.Conclusion:Subchronic aluminum exposure down-regulates the expression of NGF in the hippocampus,which may cause learning and memory impairment in offspring rats.
