The effects of electroacupuncture on oxidative stress in hippocampal neurons of neonatal rats with hypoxic ischemic brain injury
10.16557/j.cnki.1000-7547.2025.02.004
- VernacularTitle:电针对缺氧缺血性脑损伤新生大鼠海马神经元氧化应激的影响
- Author:
Xuejiu ZENG
1
;
Kun AI
1
;
Yujing ZHOU
1
;
Lifen ZHAN
1
;
Roujun LIANG
1
;
Qiangsheng DING
1
;
Hong ZHANG
1
;
Xiqin YI
1
Author Information
1. 湖南中医药大学针灸推拿与康复学院,长沙 410208
- Publication Type:Journal Article
- Keywords:
hypoxic ischemic brain injury(HIBI);
electroacupuncture(EA);
oxidative stress;
nuclear factor E2 related factor 2(Nrf2);
rat
- From:
Chinese Journal of Neuroanatomy
2025;41(2):157-164
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effects of electroacupuncture(EA)on learning and memory ability and nucle-ar factor E2 related factor 2(Nrf2),glutathione peroxidase 4(GPX4),NAD(P)H-quinone oxidoreductase 1(NQO1),reactive oxygen species(ROS)in hippocampus of neonatal rats with hypoxic-ischemic brain damage(HIBI)and its anti-oxidative stress mechanism.Methods:A total of 76 neonatal rats were randomly divided into Sham opera-tion group(Sham),HIBI model group(HIBI),electroacupuncture group(HIBI+EA),and Nrf2 inhibitor ML385 group(HIBI+EA+ML385).The neonatal rat model of HIBI was established by classical Rice method.The HIBI+EA group was treated with electroacupuncture for 30 min/d for 14 consecutive days.In the HIBI+EA+ML385 group,30 mg/kg ML385 was intraperitoneally injected at 1 h before each electroacupuncture intervention.Morris water maze test was performed on 21 days after modeling to test the learning and memory ability of neonatal rats in each group.Nissl staining and HE staining were used to observe the morphology of neurons in the hippocampal CA1 region,and DHE flu-orescent probe was used to detect the expression of ROS in the hippocampal CA1 region.The contents of Nrf2,GPX4 and NQO1 in hippocampus were detected by Western blot.Results:Compared with the Sham group,the HIBI group had severe pathological damage,a prolonged escape latency,a decreased number of platform crossings,a significantly increased expression of ROS,and a significantly decreased expression of Nrf2,GPX4,and NQO1(P<0.05).Com-pared with HIBI group,the pathological damage was significantly attenuated,the escape latency was shortened,the number of platform crossings was increased,the expression of ROS was decreased,and the expression of Nrf2,GPX4 and NQO1 was increased in HIBI+EA group(P<0.05).Compared with HIBI+EA group,the pathological damage was aggravated,the escape latency was prolonged,the number of crossing the platform was decreased,the expression of ROS was increased,and the expression of Nrf2,GPX4,and NQO1 was decreased in HIBI+EA+ML385 group(P<0.05).Conclusion:Electroacupuncture can effectively improve the learning and memory ability of hypoxic-ischemic neonatal rats,which is related to its effect in reducing oxidative stress in hippocampal neurons by regulating Nrf2 signa-ling pathway.
