Effects of hyperbaric oxygen on the apoptosis and ultrastructure of islet cells in rats with type 2 diabetes
10.3760/cma.j.issn.1009-6906.2014.04.004
- VernacularTitle:高压氧治疗对2型糖尿病大鼠胰岛细胞凋亡和超微结构的影响
- Author:
Guiqiang SHAO
1
;
Wenjie NIE
;
Xiuqin CAO
Author Information
1. 闵行区吴泾医院高压氧科, 上海,200241
- Publication Type:Journal Article
- Keywords:
Hyperbaric oxygen;
Type 2 diabetes;
Islet;
Apoptosis;
Caspase-3;
Ultrastructure
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2014;21(4):225-228
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of hyperbaric oxygen (HBO) on the apoptosis and ultrastructure of islet cells in rats with type 2 diabetes.Methods Twenty-four Goto-Kakizaki (GK) rats with fasting blood glucose of over 16.7 mmol/L were randomly assigned to the 3 groups:the model group,the metformin hydrochloride group,and the HBO group,each consisting of 8 animals.Another 8 healthy male Wistar rats were used as the control group.The control group,the model group and the HBO group were given 5 ml/kg of pure water a day.The metformin hydrochloride group had lavage of 250 mg/kg metformin hydrochloride solution a day,and the HBO group had one session of HBO treatment a day.After 3 weeks,following anesthesia with isoflurane,the pancreas tissue was taken from the rats of all the groups,some of the tissue was made into slides for electron-microscopic observation,and some of them was made into paraffin sections for staining with Hematoxylin-eosin (HE).Apoptosis of islet cells was detected with immunohistochemistry with TdT-mediated dUTP Nick-End Labeling (TUNEL),and islet cells were labeled with caspase-3 by using immunohistochemistry.Results Electron microscopy revealed that mitochondria in the animals of the model group was markedly swollen with degranulation,and vacuolation.Pathological changes inmitochondria of islet cells for the animals of the HBO group were lighter than those of the model group.Apoptosis of islet cells occurred in all the groups,with the apoptosis index (AI) of the HBO group being (1.57 ± 0.33) ％.IOD for caspase-3 positive cells was 10.22 ± 4.61.On the other hand,AI and IOD for the animals of the model group were (4.88 ± 0.51) ％ and 14.65 ± 4.8 respectively,and statistical significance could be seen,when comparisons were made between the 2 groups(P ＜ 0.05).No statistical significance could be noted,as compared with the data of AI (1.16 ± 0.31)％ and the data of IOD (5.36 ± 2.34) in the control group,and the data of AI(1.72 ± 0.14)％ and the data of IOD (9.37 ± 4.64) in the metformin hydrochloride group (P ＞ 0.05).Conclusions HBO could protect the mitochondrial membrane,alleviate mitochondrial lesion induced by hyperglycemia,and furthermore could inhibit apoptosis of islet cells in the rats with type 2 diabetes,and the expression of caspase-3 as well.Therefore,it would be beneficial for treatment of diabetes mellitus.
