Research on the effects of hyperbaric oxygen on rats behavior and stress-related indicators
10.3760/cma.j.issn.1009-6906.2014.06.002
- VernacularTitle:高压氧对大鼠行为学及应激相关指标影响的研究
- Author:
Naikeng MAI
1
;
Chen YANG
;
Ruijiao SUN
;
Yan LYU
;
Jinjing ZHAO
;
Shuyi PAN
Author Information
1. 南方医科大学研究生院, 广州,510515
- Publication Type:Journal Article
- Keywords:
Hyperbaric oxygen;
Elevated plus maze;
HPA axis;
Nitric oxide system;
TNF-α;
Rats
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2014;21(6):365-368
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between hyperbaric oxygen (HBO) and stress through observation on rats behavior and stress-related indicators.Methods Twenty Wistar rats were randomly divided into the HBO group and the control group.The HBO group was exposed to HBO at a pressure of 0.20 MPa once a day,for a succession of 21 days,and the control group was left there without HBO treatment.After one week of adaptive training,elevated plus maze test was conducted at days 1,7,14 and 21 to observe openarm (OA) time.The contents of corticotropin-releasing hormone (CRH),adrenocorticotropic hormone (ATCH),glucocorticoids (GCS),nitric oxide synthase (NOS),nitric oxide (NO) and tumor necrosis factorα (TNF-α) in serum of the animals in each group were detected by ELISA (enzyme-linked immunosorbent assay).Results HBO treatment could significantly increase the level of GCS in peripheral blood (P ＞ 0.05),but with no obvious effect on open-arm time (anxiety degree) and the expression levels of nitric oxide and TNF-α.Conclusions HBO treatment was in conformity with the general characteristics of chronic stress,but would not bring about damage to the body,suggesting that one of the mechanisms of HBO might lie in the increased tolerance of the involved tissues to stressors.
