The hypothalamic paraventricular nucleus CBS reduces blood pressure in spontaneously hypertensive rats by affecting PGC-1α
- VernacularTitle:下丘脑室旁核CBS通过影响PGC-1α降低自发性高血压大鼠的血压
- Author:
Xiaojing YU
1
;
Yanan GAO
;
Ying LI
;
Limei TU
;
Qianxi GAO
;
Yaojun SUN
;
Rongli HE
;
Yuming KANG
;
Xiaolian SHI
Author Information
- Publication Type:Journal Article
- Keywords: hypertension; hypothalamic paraventricular nucleus(PVN); cystathionine-β-synthase(CBS); peroxisome proliferator-activated receptor γ coactivator-1α(PGC-1α); oxidative stress
- From: Journal of Xi'an Jiaotong University(Medical Sciences) 2025;46(2):227-237
- CountryChina
- Language:Chinese
- Abstract: Objective To elucidate how the overexpression of cystathionine-β-synthase(CBS)plays an antihypertensive role by affecting peroxisome proliferator-activated receptor γ coactivator-1α(PGC-1α)expression.Methods The adeno-associated viruses(AAVs),ones that overexpressed CBS,and another knocked down PGC-1α,were injected into the hypothalamic paraventricular nucleus(PVN)of spontaneously hypertensive rats(SHRs).The rats'blood pressure was monitored,and the level of norepinephrine(NE)was examined by ELISA;PVN inflammatory response,oxidative stress and tyrosine hydroxylase(TH)expression were detected with RT-qPCR and immunofluorescence.Results PVN overexpression of CBS could increase the transcription level of CBS(by 3.8 times,P＜0.05)and PGC-1α(by 1.6 times,P＜0.05)in PVN of SHR.PVN overexpression of CBS could reduce blood pressure in SHR(from 177.81 mmHg to 128.77 mmHg,P＜0.001),but PVN knockdown of PGC-1αweakened such effect(from 128.77 mmHg to 152.79 mmHg,P＜0.05).PVN overexpression of CBS could alleviate PVN inflammatory response and oxidative stress,but this effect was weakened or even eliminated when knocking down PGC-1α was performed at the same time.Conclusion PVN overexpression of CBS can reduce blood pressure in SHR,and this effect may be achieved by increasing the transcriptional level of PGC-1α,alleviating PVN inflammatory response,oxidative stress,and improving sympathetic nerve excitation.
