Construction of a prognostic prediction model for skin cutaneous melanoma based on chromatin remodeling-related genes
10.11904/j.issn.1002-3070.2025.02.009
- VernacularTitle:构建基于染色质重塑相关基因的皮肤黑色素瘤预后预测模型
- Author:
Yong LI
1
;
Bingmei LIU
1
Author Information
1. 哈尔滨医科大学附属第四医院皮肤科(哈尔滨 150028)
- Publication Type:Journal Article
- Keywords:
Skin cutaneous melanoma;
Chromatin remodeling-related genes;
Prognostic genes;
Immune cell infiltration
- From:
Practical Oncology Journal
2025;39(2):134-143
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study aimed to investigate the association between chromatin remodeling-related genes(CRRGs)and overall survival(OS)of patients with skin cutaneous melanoma(SKCM),and to construct a risk score prognostic prediction mod-el.Methods Based on the TCGA and GTEx databases,the differentially expressed CRRGs in SKCM were obtained,and the progno-sis-related genes of SKCM were further screened by protein-protein interaction(PPI)network analysis,univariate Cox regression anal-ysis,and LASSO regression analysis.A risk score prognostic model was constructed based on the prognosis-related genes.According to the median of the risk score,SKCM patients were divided into the high-risk and low-risk groups.The single sample gene set enrich-ment analysis(ssGSEA)algorithm was used to evaluate the immune cell infiltration of SKCM patients between the high-and low-risk groups.Results A total of 15 hub genes were screened out through the PPI network analysis.Univariate Cox regression and LASSO regression analysis screened out 5 CRRGs associated with OS in SKCM patients,namely MMP2,MMP9,SPP1,TNFSF11,and TIMP1.A risk score was constructed based on the 5 prognostic genes,and multivariate Cox regression analysis showed that the risk score was an independent prognostic factor for SKCM patients(P<0.05).Survival analysis showed that SKCM patients in the high-risk group was shorter than that in the low-risk group(P<0.05).The results of immune cell infiltration analysis showed that there were significant differences in the infiltration ratios of 16 immune cells between the high-and low-risk groups,among which the pro-portions of activated B cells,immature B cells,effector and memory CD8+T cells and activated CD8+T cells in the high-risk group were significantly reduced(P<0.05).The proportion of CD56bright natural killer cells,CD56dim natural killer cells,γδT cells and immature dendritic cells in the high-risk group were significantly increased(P<0.05).The drug sensitivity analysis showed that there were significant differences in the sensitivity of high-risk and low-risk SKCM patients to crizotinib,erlotinib,gefitinib and vinorelbine(P<0.01).Conclusions This study constructed a prognosis model of SKCM composed of 5 CRRGs,further revealed the differences in the immune microenvironment and drug sensitivity in patients with different risk groups of SKCM,and provided an important refer-ence for personalized treatment of SKCM patients.
