Synergistic effect of formaldehyde pre-exposure on the damage to the respiratory system in rats after microbial aerosol inhalation
10.3760/cma.j.issn.1009-6906.2014.01.013
- VernacularTitle:甲醛预暴露与微生物气溶胶吸入对大鼠呼吸系统的协同损伤效应研究
- Author:
Chuanghong CHEN
1
;
Huxiang PAN
1
;
Xiongli XU
1
;
Xiaomeng REN
1
;
Qian CHEN
1
;
Haixia YUAN
1
;
Yonghua TAO
1
Author Information
1. 海军医学研究所, 上海,200433
- Publication Type:Journal Article
- Keywords:
Microbial aerosol;
Formaldehyde;
Exposure;
Respiratory system;
Synergistic damage
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2014;21(1):51-55
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the synergistic effect of formaldehyde pre-exposure on the damage to the respiratory system in rats after microbial aerosol inhalation.Methods The intoxication model was established in rats by the dynamic aerosol exposure system.Serum IgM concentration and SOD activity were measured by ELISA and luminescence assay respectively.Morphologic injury of the lung tissue was detected by HE staining.Tunnel chromogenic in-situ detection was used to detect the apoptosis of lung epithelial cells and Gram staining was performed to detect the capacity of lung epithelial cells in bacterial clearance.Results Serum IgM levels in rats increased significantly,after microbial aerosol inhalation,when compared with that of the control group,with the serum IgM levels of the formaldehyde combined with microbial aerosol inhalation group increased more significantly [(0.35 ±0.09) g/L].The serum SOD activity of the experimental groups all increased,with the level of the formaldehyde combined with microbial aerosol inhalation group [(2.22 ± 0.25) × 106 U/L] being significantly higher than that of the 2 other groups [(1.50 ±0.37) × 106 U/L] and [(1.58 ± 0.34) × 106 U/L].Morphological observation showed that widened alveolar septum,interstitial edema and inflammatory cell infiltration could be clearly noted in the lung tissue of the 3 experimental groups,with the pathological changes in the formaldehyde combined with microbial aerosol inhalation group being most significant.Tunnel detection also indicated that the nnmber of apoptotic cells in the lung tissue for the formaldehyde combined with microbial aerosol inhalation group increased significantly.Gram staining showed that the number of residue bacteria in the rat bronchiole for the rats of the formaldehyde combined with microbial aerosol inhalation group after 2-hour air intervention was significantly more than that of the simple bacteria exposure group.Conclusions Formaldehyde pre-exposure combined with microbial aerosol inhalation could induce synergistic damage to the rat lung tissue and decrease the capacity of the lung tissue to clear out heterogeneous pollutants.
