Optimization of the Extraction Process of Changyan Heji Ⅱ Based on UPLC-Q-TOF-MS/MS Combined with Response Surface Method and Principal Component Analysis
10.14148/j.issn.1672-0482.2025.0501
- VernacularTitle:基于UPLC-Q-TOF-MS/MS结合响应面法与主成分分析优化肠炎合剂Ⅱ提取工艺
- Author:
Shulin WANG
1
;
Jing SHANG
;
Wenjun LIU
;
Zerong CAI
;
Mengyu QIAN
;
Xiaoxin HU
;
Liang CAO
;
Zhenz-hong WANG
;
Wei XIAO
Author Information
1. 中药制药过程控制与智能制造技术全国重点实验室(江苏康缘药业股份有限公司/南京中医药大学),江苏 南京 210000;江苏康缘药业股份有限公司,江苏 连云港 222000
- Publication Type:Journal Article
- Keywords:
extraction process;
Box-Behnken design-response surface methodology;
principal component analysis;
puerarin;
gly-cyrrhizic acid;
costunolide;
paeoniflorin
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2025;41(4):501-512
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To establish a extraction process of Changyan Heji Ⅱ(CYHJ-Ⅱ)based on UPLC-Q-TOF-MS/MS technology combined with response surface analysis,and to optimize the extraction process.METHODS The chemical components in CYHJ-Ⅱ were qualitatively analyzed by UPLC-Q-TOF-MS/MS technology,and the chemical components with good linear relation-ship in mass spectrometry response were selected as process investigation indicators;the extraction process parameters(water addition amount,extraction time and soaking time)were investigated by Box-Behnken design;the comprehensive score was obtained by princi-pal component analysis(PCA),and the optimal process was determined by the comprehensive score combined with response surface a-nalysis.RESULTS Through qualitative analysis,110 components were inferred and identified from CYHJ-Ⅱ,including 2 organic acids,82 flavonoids,13 terpenoids,and 13 alkaloids.Based on the results of qualitative analysis,48 index components with good lin-ear relationships were derived by UPLC-Q-TOF-MS/MS combined with Masshunter mass spectrometry data analysis software.PCA was performed and the comprehensive score was calculated.Response surface analysis was performed with the comprehensive score as an indicator.The optimal extraction process obtained by combining the response surface prediction results and actual production was:soaking for 45 min,8 times the amount of solvent,2 extractions,each time for 120 min.CONCLUSION This study provides a new idea for the investigation of the extraction process of traditional Chinese medicine compound prescriptions and expands a new method for the development of traditional Chinese medicine compound prescriptions.
