Analysis of Chemical Components of Suanzaoren Decoction and Components Absorbed into Blood of Female Rats Based on UHPLC-Q Exactive Orbitrap-MS
10.14148/j.issn.1672-0482.2025.0375
- VernacularTitle:基于UHPLC-Q Exactive Orbitrap-MS分析酸枣仁汤化学成分及雌性大鼠入血成分
- Author:
Ziyang KONG
1
;
Liang WU
;
Wen LI
;
Jie HAN
;
Chenmin SHENG
;
Yuheng LIAN
;
Lingdong MENG
;
Yunan ZHAO
;
Yaoyao BIAN
Author Information
1. 南京中医药大学针灸推拿学院·养生康复学院,江苏 南京 210023
- Publication Type:Journal Article
- Keywords:
Suanzaoren Decoction;
female rats;
blood-absorbed components;
UHPLC-Q Exactive Orbitrap-MS;
metabolites
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2025;41(3):375-382
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To analyze the blood components of Suanzaoren Decoction after oral administration using UHPLC-Q Exactive Orbitrap-MS technology.METHODS Female Sprague-Dawley(SD)rats were used as experimental subjects,and Suanza-oren Decoction was administered orally.Serum samples were collected,and the aqueous extract of Suanzaoren Decoction and the serum were analyzed using UHPLC-Q Exactive Orbitrap-MS technology to identify the prototype components and metabolites absorbed into the blood by comparing and analyzing with the LuMet-TCM database.RESULTS It showed that a total of 458 components were iden-tified in the aqueous extract of Suanzaoren Decoction,and 26 chemical components were identified in the blood,including 23 prototype components and 3 metabolites.CONCLUSION The prototype components absorbed into the blood discovered in this study may be the active ingredients of Suanzaoren Decoction,providing a reference for the research on the pharmacodynamic material basis of Suanza-oren Decoction.
